Cui C B, Kakeya H, Okada G, Onose R, Osada H
Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
J Antibiot (Tokyo). 1996 Jun;49(6):527-33. doi: 10.7164/antibiotics.49.527.
Two novel diketopiperazines named tryprostatins A (1) and B (2) and a new natural product belonging to the diketopiperazine series, designated as demethoxyfumitremorgin C (3), together with four known diketopiperazines, fumitremorgin C (4), 12,13-dihydroxyfumitremorgin C (5), fumitremorgin B (6) and verruculogen (7), were isolated from the fermentation broth of Aspergillus fumigatus BM939 by the combined use of solvent extraction, silica gel column chromatography, preparative TLC and repeated-preparative HPLC. The diketopiperazines showed an inhibitory activity on the cell cycle progression of mouse tsFT210 cells in the M phase with the MIC values of 16.4 microM (1), 4.4 microM (2), 0.45 microM (3), 4.1 microM (4), 60.8 microM (5), 26.1 microM (6) and 12.2 microM (7), respectively.
通过溶剂萃取、硅胶柱色谱、制备薄层层析和反复制备高效液相色谱联用的方法，从烟曲霉BM939的发酵液中分离出两种新型二酮哌嗪，分别命名为曲前列他汀A（1）和B（2），以及一种属于二酮哌嗪系列的新天然产物，命名为去甲氧基烟曲霉震颤素C（3），同时还分离出四种已知的二酮哌嗪，烟曲霉震颤素C（4）、12,13 - 二羟基烟曲霉震颤素C（5）、烟曲霉震颤素B（6）和疣孢菌素（7）。这些二酮哌嗪对处于M期的小鼠tsFT210细胞的细胞周期进程具有抑制活性，其最小抑菌浓度（MIC）值分别为16.4微摩尔/升（1）、4.4微摩尔/升（2）、0.45微摩尔/升（3）、4.1微摩尔/升（4）、60.8微摩尔/升（5）、26.1微摩尔/升（6）和12.2微摩尔/升（7）。
