Maillard J Y, Beggs T S, Day M J, Hudson R A, Russell A D
Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
J Appl Bacteriol. 1996 Jun;80(6):605-10. doi: 10.1111/j.1365-2672.1996.tb03264.x.
The coliphage K1-5 has been used in an automated assay to monitor the viricidal activity of various disinfectants. This indirect assay based on the spectrophotometric reading of the lysis of the host cell (Escherichia coli D837) produced encouraging results and was faster than the overlay counting method (previously studied) which relies on plaque formation. However, differences in sensitivity towards some disinfectants were observed between the two methods.
大肠杆菌噬菌体K1-5已被用于一种自动化检测中,以监测各种消毒剂的杀病毒活性。这种基于对宿主细胞(大肠杆菌D837)裂解的分光光度读数的间接检测产生了令人鼓舞的结果,并且比依赖噬菌斑形成的(之前研究过的)覆盖计数法更快。然而,两种方法之间观察到了对某些消毒剂敏感性的差异。