Maillard J Y, Hann A C, Beggs T S, Day M J, Hudson R A, Russell A D
Welsh School of Pharmacy, University of Wales College of Cardiff.
J Med Microbiol. 1995 Jun;42(6):415-20. doi: 10.1099/00222615-42-6-415.
Electronmicroscopy was used to observe morphological changes of the Pseudomonas aeruginosa PA0 bacteriophage F116 when treated with various biocides commonly used as antibacterial and antifungal agents. Because of its large size (145 nm) and its organised structure (an isometric head and a tail), it was possible to classify structural damage into eight categories. The morphological changes induced depended on the type of biocide used and its concentration. Glutaraldehyde increased the number of phages with empty heads. Peracetic acid and phenol altered the appearance of the viral genome packaged inside the head, produced fractured heads, and damaged the tail. Peracetic acid also induced folding of the phage heads. The alcohols tested also altered the head structure. Cetylpyridinium chloride induced mainly fractured head damage. Chlorhexidine had little effect on the structure of F116.
利用电子显微镜观察铜绿假单胞菌PA0噬菌体F116在用各种常用的抗菌和抗真菌生物杀灭剂处理时的形态变化。由于其尺寸较大（145纳米）且结构有组织（等轴头和尾），有可能将结构损伤分为八类。所诱导的形态变化取决于所用生物杀灭剂的类型及其浓度。戊二醛增加了头部为空的噬菌体数量。过氧乙酸和苯酚改变了包装在头部内的病毒基因组的外观，产生了破裂的头部，并损坏了尾部。过氧乙酸还诱导噬菌体头部折叠。所测试的醇类也改变了头部结构。十六烷基氯化吡啶主要诱导头部破裂损伤。洗必泰对F116的结构影响很小。
