Martins E, Mestriner M A, Contel E P
Biochem Genet. 1977 Apr;15(3-4):357-66. doi: 10.1007/BF00484466.
A polymorphic system of ADH isozymes is described in the honeybee Apis mellifera. Three and six different electrophoretic patterns were found, respectively, in drone and worker pupae analysis. The data indicate that the ADH isozymes are controlled by three alleles, Adh-1(1), Adh-1(2), and Adh-1(3). The frequency of the Adh-1 alleles is different in two analyzed subspecies, Apis mellifera adansonii (African bees) and Apis mellifera ligustica (Italian bees). In the African bees, the frequencies are 0.256 and 0.697 for Adh-1(1) and Adh-1(2), respectively. In the Italian bees, these values are shown to be 0.902 and 0.098, respectively. The allele Adh-1(3) was not detected in the Italian bee population. The effect of NAD on the resolution of this system was investigated, and only one region of ADH activity was obtained in drone pupae analysis when NAD was used in the gels. However, two different regions of activity were observed in the same samples, in the absence of the coenzyme. ADH activity was not detected in young larvae, but it increased to a maximum in prepupal and white-eyed pupal phases. It then declined progressively to total absence in the emerging bees.
在蜜蜂意大利蜜蜂中描述了一种乙醇脱氢酶（ADH）同工酶的多态系统。在雄蜂和工蜂蛹分析中分别发现了三种和六种不同的电泳模式。数据表明，ADH同工酶由三个等位基因Adh-1(1)、Adh-1(2)和Adh-1(3)控制。在两个被分析的亚种——非洲蜜蜂（Apis mellifera adansonii）和意大利蜜蜂（Apis mellifera ligustica）中，Adh-1等位基因的频率不同。在非洲蜜蜂中，Adh-1(1)和Adh-1(2)的频率分别为0.256和0.697。在意大利蜜蜂中，这些值分别为0.902和0.098。在意大利蜜蜂群体中未检测到等位基因Adh-1(3)。研究了NAD对该系统分辨率的影响，当在凝胶中使用NAD时，在雄蜂蛹分析中仅获得了一个ADH活性区域。然而，在没有辅酶的情况下，在相同样品中观察到了两个不同的活性区域。在幼虫期未检测到ADH活性，但在预蛹期和白眼蛹期增加到最大值。然后它逐渐下降，在羽化出的蜜蜂中完全消失。
