肝糖原的分子大小与形状。
The molecular size and shape of liver glycogen.
作者信息
Geddes R, Harvey J D, Wills P R
出版信息
Biochem J. 1977 May 1;163(2):201-9. doi: 10.1042/bj1630201.
Abstract
The molecular-weight distribution of liver glycogen has been established from the analysis of sedimentation rates of fractions separated on sucrose density gradients and from the direct measurement of the diffusion coefficients of these fractions by laser-intensity-fluctuation spectroscopy. Hydrodynamic studies indicated that all fractions of glycogen of mol.wt.exceeding 25x10(6) had about 1.1 g of water per g of polysaccharide associated with them. The hydration and hydrodynamic behaviour of all fractions of mol.wt. exceeding 25x10(6) was similar, whereas smaller fractions behaved anomalously, indicating a substantially different overall structure.
摘要
通过对在蔗糖密度梯度上分离的级分沉降速率的分析以及利用激光强度波动光谱法直接测量这些级分的扩散系数，已确定了肝脏糖原的分子量分布。流体动力学研究表明，所有分子量超过25×10⁶的糖原级分，每克多糖约结合1.1克水。所有分子量超过25×10⁶的级分的水合作用和流体动力学行为相似，而较小的级分表现异常，表明其整体结构存在显著差异。
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本文引用的文献
1
VISCOSITY AND THE SHAPE OF PROTEIN MOLECULES.粘度与蛋白质分子的形状
Science. 1940 Aug 9;92(2380):132-3. doi: 10.1126/science.92.2380.132.
2
THE SIZE AND SHAPE OF RAT LIVER GLYCOGEN PREPARED BY PHENOL EXTRACTION.
Isr J Med Sci. 1965 Jan;1:26-42.
3
A COMPARISON OF PRODUCTS OBTAINED BY VARIOUS PROCEDURES USED FOR THE EXTRACTION OF GLYCOGEN.通过各种用于提取糖原的方法所获得的产物的比较。
J Biol Chem. 1964 Dec;239:4021-6.
4
RECENT STUDIES ON THE STRUCTURE OF TOBACCO MOSAIC VIRUS.烟草花叶病毒结构的近期研究
Adv Protein Chem. 1963;18:1-35. doi: 10.1016/s0065-3233(08)60267-3.
5
[Morphology of glycogen. Electron microscopic study of the negative stains of particulate glycogen].[糖原的形态学。颗粒状糖原负染的电子显微镜研究]
J Ultrastruct Res. 1962 Apr;6:141-63. doi: 10.1016/s0022-5320(62)90050-3.
6
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
7
Further studies on high molecular weight liver glycogen.关于高分子量肝糖原的进一步研究。
Arch Biochem Biophys. 1966 Feb;113(2):265-72. doi: 10.1016/0003-9861(66)90186-x.
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Natl Cancer Inst Monogr. 1966 Jun;21:219-39.
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Arch Biochem Biophys. 1970 Nov;141(1):228-35. doi: 10.1016/0003-9861(70)90126-8.
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