Hamano M, Kuroda Y, Saitoh Y
Department of Surgery, Kobe University School of Medicine.
Kobe J Med Sci. 1996 Apr;42(2):93-104.
To get a successful pancreas transplantation in a rat model, we performed heterotopic pancreas transplantation with non-suture cuff technique to neck vessels. As the graft was placed in the neck, we could avoid the clamping of systemic circulation which is essential but detriment to the rat during vascular anastomosis in intraabdominal grafting. The diameter of the portal vein was enlarged to prevent outflow block after transplantation. The cuff was fixed by fat clay during anastomosis to perform vascular anastomosis easily. As a result the success rates was 16/20, 80%. 16 rats survived more than 30 days and maintained serum glucose level within 200 mg/dl throughout the experiment. The heterotopic pancreas transplantation using non-suture cuff technique to the neck vessels is safe, reliable and recommended as the experimental model using the rat.
为了在大鼠模型中成功进行胰腺移植，我们采用非缝合袖套技术将胰腺异位移植到颈部血管。由于移植物置于颈部，我们可以避免在腹腔内移植时血管吻合过程中对大鼠至关重要但有害的全身循环夹闭。门静脉直径扩大以防止移植后流出道阻塞。吻合时用脂肪泥固定袖套以便轻松进行血管吻合。结果成功率为16/20，即80%。16只大鼠存活超过30天，并且在整个实验过程中血清葡萄糖水平维持在200mg/dl以内。采用非缝合袖套技术将胰腺异位移植到颈部血管是安全、可靠的，推荐作为大鼠实验模型。
