通过艾姆斯试验评估某些煤衍生腐殖化合物的致突变活性。
Mutagenic activity of some coal-derived humic compounds evaluated by the Ames test.
作者信息
Bernacchi F, Ponzanelli I, Barale R, Loprieno N
机构信息
Dipartimento Scienze dell'Ambiente e del Territorio, Università degli Studi di Pisa, Italy.
出版信息
Mutat Res. 1996 Jul 10;369(1-2):107-12. doi: 10.1016/s0165-1218(96)90054-3.
Two coal-derived humic substances (Sulcis and South Africa, Eniricerche, Italy) have been evaluated for their mutagenic activity on TA98 and TA100 Salmonella typhimurium strains, either in presence or in absence of metabolic activation (S9). Both compounds showed no effect on the two strains, as observed with natural humic acid (Fluka). After chlorination, coal-derived humic acids induced a strong dose-related increase in the number of revertants on TA100 without S9, whose extent was directly proportional to the chlorination ratios. Such effect was completely suppressed when a sodium thiosulfate solution (10%) was added at the end of the chlorination period (about 90 h). The analogies with natural humic acid mutagenicity are discussed.
已对两种煤衍生腐殖质（意大利埃尼里切公司在意大利苏尔西和南非获取）在有或无代谢活化剂（S9）的情况下对鼠伤寒沙门氏菌TA98和TA100菌株的致突变活性进行了评估。正如天然腐殖酸（Fluka）所显示的那样，这两种化合物对这两种菌株均无影响。氯化后，煤衍生腐殖酸在无S9的情况下使TA100上的回复突变体数量呈强烈的剂量相关增加，其增加程度与氯化比例直接成正比。当在氯化期结束时（约90小时）加入硫代硫酸钠溶液（10%）时，这种效应完全被抑制。文中讨论了与天然腐殖酸致突变性的相似之处。
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