通过艾姆斯试验评估光叶朴（榆科）提取物对鼠伤寒沙门氏菌TA98和TA100菌株碱基对交换和移码突变的抗诱变作用。

Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test.

作者信息

Akin Duygu, Durak Yusuf, Uysal Ahmet, Gunes Erdogan, Aladag Mustafa Onur

机构信息

a Meram Faculty of Medicine, Department of Anatomy , Necmettin Erbakan University , Konya , Turkey .

b Faculty of Science, Department of Biology , Selcuk University , Konya , Turkey , and.

出版信息

Drug Chem Toxicol. 2016;39(3):312-21. doi: 10.3109/01480545.2015.1121273. Epub 2016 Jan 7.

DOI:10.3109/01480545.2015.1121273

PMID:26739110

Abstract

CONTEXT

Celtis glabrata is used in Turkey for the treatment of various health disorders.

OBJECTIVE

The acetone, chloroform, ethanol, and methanol extracts of C. glabrata leaf, fruit, and seed were investigated to evaluate their antimutagenic activities.

MATERIAL AND METHODS

The antimutagenicity of these extracts was determined by Ames test against mutagens (4-nitro-O-phenylenediamine, 2-aminofluorene (2-AF), and sodium azide (SA)). The extracts were used at concentrations between 5 and 0.005 mg/plate.

RESULTS

The ethanol extracts of leaves exhibited strong antimutagenicity (70%) against 2-AF with S9 at 5 mg/plate on TA98. But methanol (61%, 53%) and acetone (53%, 52%) also revealed strong inhibition rates at concentrations of ≥ 0.5 mg/plate. Among the extracts, the highest activity (96%) was obtained from acetone extract against SA without S9, followed by chloroform extract (91%) at a dose of 5 mg/plate on TA100 with S9. Ethanol (without S9) and chloroform (with S9) extracts showed strong antimutagenicity at all doses. Exception of chloroform and acetone (without S9), all fruit extracts (with/without S9) manifested strong antimutagenicity at doses of ≥ 0.5 mg/plate on TA98 strain. Ethanol extracts revealed 68% inhibition against 2-AF on TA98. Acetone and ethanol extracts manifested 84% and 82% inhibition against SA on TA100, respectively. All the extracts of seeds revealed strong inhibition against 2-AF at ≥ 0.5 mg/plate doses on TA98, but acetone extract showed excellent antimutagenicity (94%). Moreover, the chloroform (74, 73, 63, 54%), acetone (74, 72, 70, 65%) and methanol (74, 67, 63, 61%) extracts of seeds revealed strong antimutagenic activity on TA100 against SA with S9.

DISCUSSION AND CONCLUSION

This plant may be natural source of antimutagenic agents.

摘要

背景

在土耳其，光叶朴被用于治疗多种健康疾病。

目的

研究光叶朴叶、果实和种子的丙酮、氯仿、乙醇和甲醇提取物，以评估它们的抗诱变活性。

材料与方法

通过艾姆斯试验测定这些提取物对诱变剂（4-硝基邻苯二胺、2-氨基芴（2-AF）和叠氮化钠（SA））的抗诱变性。提取物的使用浓度为5至0.005毫克/平板。

结果

叶的乙醇提取物在TA98平板上，以5毫克/平板的浓度与S9一起使用时，对2-AF表现出较强的抗诱变性（70%）。但甲醇（61%，53%）和丙酮（53%，52%）在浓度≥0.5毫克/平板时也显示出较高的抑制率。在这些提取物中，丙酮提取物在无S9的情况下对SA的活性最高（96%），其次是氯仿提取物（91%），在TA100平板上以5毫克/平板的剂量与S9一起使用。乙醇（无S9）和氯仿（有S9）提取物在所有剂量下均表现出较强的抗诱变性。除氯仿和丙酮（无S9）外，所有果实提取物（有/无S9）在TA98菌株上，以≥0.5毫克/平板的剂量表现出较强的抗诱变性。乙醇提取物在TA98上对2-AF的抑制率为68%。丙酮和乙醇提取物在TA100上对SA的抑制率分别为84%和82%。种子提取物在TA98上，以≥0.5毫克/平板的剂量对2-AF均表现出较强的抑制作用，但丙酮提取物表现出优异的抗诱变性（94%）。此外，种子的氯仿（74%，73%，63%，54%）、丙酮（74%，72%，70%，65%）和甲醇（74%，67%，63%，61%）提取物在TA100上，对有S9的SA表现出较强的抗诱变活性。