Gerald C, Walker M W, Criscione L, Gustafson E L, Batzl-Hartmann C, Smith K E, Vaysse P, Durkin M M, Laz T M, Linemeyer D L, Schaffhauser A O, Whitebread S, Hofbauer K G, Taber R I, Branchek T A, Weinshank R L
Synaptic Pharmaceutical Corporation, Paramus, New Jersey 07652-1431, USA.
Nature. 1996 Jul 11;382(6587):168-71. doi: 10.1038/382168a0.
Neuropeptide Y (NPY) is a powerful stimulant of food intake and is proposed to activate a hypothalamic 'feeding' receptor distinct from previously cloned Y-type receptors. This receptor was first suggested to explain a feeding response to NPY and related peptides, including NPY2-36, that differed from their activities at the Y1 receptor. Here we report the expression cloning of a novel Y-type receptor from rat hypothalamus, which we name Y5. The complementary DNA encodes a 456-amino-acid protein with less than 35% overall identity to known Y-type receptors. The messenger RNA is found primarily in the central nervous system, including the paraventricular nucleus of the hypothalamus. The extent to which selected peptides can inhibit adenylate cyclase through the Y5 receptor and stimulate food intake in rats correspond well. Our data support the idea that the Y5 receptor is the postulated 'feeding' receptor, and may provide a new method for the study and treatment of obesity and eating disorders.
神经肽Y（NPY）是一种强大的食欲刺激剂，有人提出它能激活一种不同于先前克隆的Y型受体的下丘脑“进食”受体。最初提出这种受体是为了解释对NPY及相关肽（包括NPY2 - 36）的进食反应，这种反应不同于它们在Y1受体上的活性。在此我们报告从大鼠下丘脑克隆出一种新型Y型受体，我们将其命名为Y5。互补DNA编码一种456个氨基酸的蛋白质，与已知Y型受体的总体同源性不到35%。信使核糖核酸主要存在于中枢神经系统，包括下丘脑室旁核。所选肽通过Y5受体抑制腺苷酸环化酶的程度与刺激大鼠进食的程度相符。我们的数据支持Y5受体就是假定的“进食”受体这一观点，并且可能为肥胖症和饮食失调的研究与治疗提供一种新方法。
