Petropoulos C J, Givol I, Hughes S H
ABL-Basic Research Program, NCI-Frederick Cancer Research and Developmental Center, Frederick, Maryland 21702-1201, USA.
Oncogene. 1996 Jun 20;12(12):2611-21.
To gain a more complete understanding of c-myc regulation in chickens, we have completed the structural characterization of the chicken c-myc gene and have begun to investigate c-myc transcription and protein expression. A comparison of c-myc structure and expression between mammals and birds presents an enigma: there are striking similarities in the pattern of gene expression in the absence of obvious sequence similarities in the controlling elements. We have begun to investigate c-myc and v-myc function using retroviral vectors that differ solely in the Myc proteins that they express. We show that while the overexpression of the smaller c-Myc protein is sufficient to induce morphological transformation in chicken embryo fibroblasts, overexpression of v-Myc provides a stronger signal for cells to enter the cell cycle and is a more potent inducer of apoptosis than c-Myc.
为了更全面地了解鸡体内c-myc的调控机制，我们已完成鸡c-myc基因的结构特征分析，并开始研究c-myc的转录及蛋白表达情况。对哺乳动物和鸟类的c-myc结构及表达进行比较时发现了一个谜题：在控制元件中缺乏明显序列相似性的情况下，基因表达模式却存在显著相似性。我们已开始使用仅在表达的Myc蛋白上存在差异的逆转录病毒载体来研究c-myc和v-myc的功能。我们发现，虽然较小的c-Myc蛋白的过表达足以诱导鸡胚成纤维细胞发生形态转化，但v-Myc的过表达为细胞进入细胞周期提供了更强的信号，并且与c-Myc相比，它是一种更有效的凋亡诱导剂。
