Parfenov V V, Temarinova Iu N, Balandin I G
Biull Eksp Biol Med. 1977 Apr;83(4):448-9.
Different means of attenuating the leukemogenic activity were studied comparatively with a continuous strain of bone marrow cells derived from mice infected with Rauscher leukemia virus. Treatment of leukemic cells with neuraminidase, like their cultivation at suboptimal temperature resulted in a complete loss of leukemogenic activity, as evidenced by 100% survival of experimental animals and the absence of splenomegaly. In parallel experiments with concanavallin A and 5-bromdeoxyuridine treatment, development of splenomegaly was retarded and lethality of the animals reduced by 70 and 20%, rnd 20%, respectively. These results permit to raise the problem of using the above-memtioned methods for the attenuation of leukemogenic activity as an approach to prepare material with the immunizing activity.
利用来自感染劳斯氏白血病病毒的小鼠的连续骨髓细胞系，对不同的白血病致病毒性减弱方法进行了比较研究。用神经氨酸酶处理白血病细胞，就如同在次优温度下培养它们一样，会导致白血病致病毒性完全丧失，实验动物100%存活且无脾肿大即可证明这一点。在伴刀豆球蛋白A和5-溴脱氧尿苷处理的平行实验中，脾肿大的发展受到抑制，动物的致死率分别降低了70%和20%。这些结果使得人们能够提出一个问题，即使用上述方法减弱白血病致病毒性作为一种制备具有免疫活性物质的途径。
