Idusogie E, Rosen E, Geng J P, Carmeliet P, Collen D, Castellino F J
Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556, USA.
Thromb Haemost. 1996 Mar;75(3):481-7.
The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a lambda Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.
从小鼠肝脏mRNA构建的λZap cDNA文库中分离并重建了编码小鼠凝血因子VII（mfVII）的cDNA。该cDNA含有1903个核苷酸，跨越5'-翻译起始密码子上游15个碱基、1338个核苷酸的开放阅读框、第一个终止密码子下游550个核苷酸以及3' poly(A)尾。翻译产物由一个41个氨基酸的信号/前肽区域和一个405个残基的成熟蛋白组成。后者与人类、兔子、牛、恒河猴和犬类fVII的高度同源。hfVII的所有蛋白结构域在mfVII中严格保守。
