Pallister J A, Sheppard M
CSIRO Division of Animal Health, Vic., Australia.
Vet Microbiol. 1996 Jan;48(1-2):155-63. doi: 10.1016/0378-1135(95)00140-9.
Restriction enzyme studies have been used to divide the fowl adenoviruses (FAV) into 5 groups - A, B,C,D and E. More detailed restriction enzyme studies of a series of group E FAV field isolates showed that these methods could differentiate between mildly and hypervirulent FAV belonging to this group. We have mapped the genomes of the hypervirulent (CFA 40) and two of the mildly virulent FAV (CFA 44 and CFA 3), using 11 different restriction enzymes: HindIII, BglII, XbaI, NdeI, SpeI, DraI, NotI, StuI, NheI, SfiI, and AvrII. Comparison of the three maps showed that the CFA 3 genome was approximately 3.5 kb smaller than that of CFA 44 and CFA 40. This size difference was discounted as a likely cause of the reduced pathogenicity of CFA 3 as the other mildly virulent virus, CFA 44, was the same size as the hypervirulent CFA 40. Other variations between the three viruses occurred in the region of the hexon and 100 K genes, but further studies are required to determine the significance of these variations in pathogenicity.
限制性内切酶研究已被用于将禽腺病毒（FAV）分为5组——A、B、C、D和E组。对一系列E组FAV野外分离株进行的更详细的限制性内切酶研究表明，这些方法可以区分属于该组的温和型和超强毒力型FAV。我们使用11种不同的限制性内切酶（HindIII、BglII、XbaI、NdeI、SpeI、DraI、NotI、StuI、NheI、SfiI和AvrII）绘制了超强毒力型（CFA 40）和两种温和毒力型FAV（CFA 44和CFA 3）的基因组图谱。对这三张图谱的比较表明，CFA 3基因组比CFA 44和CFA 40的基因组大约小3.5 kb。由于另一种温和毒力型病毒CFA 44与超强毒力型CFA 40大小相同，因此这种大小差异被排除为CFA 3致病性降低的可能原因。这三种病毒之间的其他差异发生在六邻体和100K基因区域，但需要进一步研究以确定这些差异在致病性方面的意义。
