Mu G, Dong Y, Huang G
Department of Agronomy, Hebei Agriculture University, Baoding.
Wei Sheng Wu Xue Bao. 1995 Oct;35(5):322-6.
The results of the interspecific protoplast fusion between B. thuringensis sub. kurstaki Bt-3701 which has pesticide ability, and B. megaterium var. phosphaticum Bm-107 which has decomposing phosphate activity, were reported. High frequency of protoplast formation and regeneration was obtained with 4h activated Bm-107 treated by 100 micrograms/ml lysozyme, and with 2h activated Bt-3701 treated by 3% glycin and mild temperature. Using 40% PEG and 5% nascent Ca2+ to treat the parential protoplast mixture for 3 min at 37 degrees C, 4 stable fusants were obtained. Biological tests show that they have both pesticide ability and decomposing phosphate activity, but which are weaker than that of parential strains.
报道了具有杀虫能力的苏云金芽孢杆菌库斯塔克亚种Bt - 3701与具有解磷活性的巨大芽孢杆菌变种Bm - 107之间种间原生质体融合的结果。用100微克/毫升溶菌酶处理活化4小时的Bm - 107，以及用3%甘氨酸和温和温度处理活化2小时的Bt - 3701，可获得较高频率的原生质体形成和再生。使用40%聚乙二醇（PEG）和5%新生Ca2 +在37℃下处理亲本原生质体混合物3分钟，获得了4株稳定的融合体。生物学测试表明，它们兼具杀虫能力和解磷活性，但比亲本菌株的相应能力弱。
