巨大芽孢杆菌原生质体膜的分离与分析
Isolation and analysis of the protoplast membrane of Bacillus megaterium.
作者信息
Yudkin M D
出版信息
Biochem J. 1966 Mar;98(3):923-8. doi: 10.1042/bj0980923.
Abstract
- Optimum conditions were found for the lysis of Bacillus megaterium KM by lysozyme. The age of culture, density of suspension and concentration of lysozyme affected the rate of lysis. 2. Protoplast membranes were isolated by centrifugation of lysates and were exhaustively washed. 3. Treatment with chloroform removed some lipid from the membranes, but about half of the total membrane lipid could be extracted only after partial acid hydrolysis. 4. The defatted membranes consisted of protein together with variable amounts of RNA; carbohydrate was almost absent. 5. Lipid accounted for 23% of the weight of the membrane, and included both neutral lipid and phospholipid. In both classes, branched-chain C(15) acids made up about 80% of the total fatty acid. 6. The phospholipid was a kephalin, and contained small quantities of several amino acids.
摘要
- 发现了溶菌酶裂解巨大芽孢杆菌KM的最佳条件。培养物的菌龄、悬浮液密度和溶菌酶浓度会影响裂解速率。2. 通过对裂解物进行离心分离得到原生质体膜，并进行彻底洗涤。3. 用氯仿处理可去除膜中的一些脂质，但只有经过部分酸水解后，才能提取出约一半的总膜脂质。4. 脱脂膜由蛋白质和不同量的RNA组成；几乎不含碳水化合物。5. 脂质占膜重量的23%，包括中性脂质和磷脂。在这两类脂质中，支链C(15)酸约占总脂肪酸的80%。6. 磷脂是脑磷脂，含有少量几种氨基酸。
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本文引用的文献
1
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Biochem J. 1949;44(4):402-7. doi: 10.1042/bj0440402.
2
Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall.糖类的滤纸分配色谱法：1. 概述及其在苹果汁、蛋清和绵羊胎儿血液中糖类定性分析的应用。附R.G.韦斯托尔的注释
Biochem J. 1948;42(2):238-50. doi: 10.1042/bj0420238.
3
The estimation of phosphorus.磷的测定
Biochem J. 1940 Jun;34(6):858-65. doi: 10.1042/bj0340858.
4
Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates.酵母代谢的研究。I. 细胞碳水化合物的分级分离与微量测定。
Biochem J. 1952 Jan;50(3):298-303. doi: 10.1042/bj0500298.
5
The purine and pyrimidine composition of deoxypentose nucleic acids.脱氧戊糖核酸的嘌呤和嘧啶组成。
Biochem J. 1951 May;48(5):584-90. doi: 10.1042/bj0480584.
6
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
7
Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme.细菌细胞壁的研究。VI. 细胞壁组成与对溶菌酶的敏感性。
Biochim Biophys Acta. 1960 Apr 22;39:398-407. doi: 10.1016/0006-3002(60)90191-8.
8
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J Mol Biol. 1965 May;12:193-204. doi: 10.1016/s0022-2836(65)80293-5.
9
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Biochim Biophys Acta. 1963 Oct 8;78:136-47. doi: 10.1016/0006-3002(63)91619-6.
10
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J Bacteriol. 1963 Jan;85(1):168-76. doi: 10.1128/jb.85.1.168-176.1963.
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