Pöhlmann R, Philippsen P
Institute for Applied Microbiology, University of Basel, Switzerland.
Yeast. 1996 Mar 30;12(4):391-402. doi: 10.1002/(SICI)1097-0061(19960330)12:4%3C391::AID-YEA921%3E3.0.CO;2-N.
A sequence of 31431 bp located on the left arm of chromosome (chr.) XIV from Saccharomyces cerevisiae was analysed. A total of 18 open reading frames (ORFs) could be identified. Twelve ORFs are new, two of which are most likely ribosomal protein genes, leaving ten ORFs of unknown function. Nine of the 18 ORFs show either at least 20% overall amino acid identity or significant regional homology to other S. cerevisiae ORFs. Additionally, six of these nine ORFs have homologues of similar size and the same transcriptional orientation within a stretch of 50 kb on chromosome IX. The degree of homology ranges from 90% overall identity to 23% in 375 amino acids. The homologues on chromosome IX are grouped in two blocks that are separated by relatively long ORFs. This is the first example of a multi-gene duplication in S. cerevisiae not linked to a centromere or subtelomere region.
对位于酿酒酵母十四号染色体（chr.）左臂上的一段31431 bp序列进行了分析。总共鉴定出18个开放阅读框（ORF）。其中12个ORF是新的，其中两个很可能是核糖体蛋白基因，剩下10个ORF功能未知。18个ORF中的9个与其他酿酒酵母ORF显示出至少20%的总体氨基酸同一性或显著的区域同源性。此外，这9个ORF中的6个在九号染色体上50 kb的区域内有大小相似且转录方向相同的同源物。同源性程度从总体同一性的90%到375个氨基酸中的23%不等。九号染色体上的同源物分为两个区域，由相对较长的ORF隔开。这是酿酒酵母中多基因重复但不与着丝粒或亚端粒区域相连的首个例子。
