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对酿酒酵母XI号染色体左臂上51.6千碱基的序列进行测序和分析，发现了23个开放阅读框，其中包括FAS1基因。

Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene.

作者信息

Wiemann S, Voss H, Schwager C, Rupp T, Stegemann J, Zimmermann J, Grothues D, Sensen C, Erfle H, Hewitt N

机构信息

Biochemical Instrumentation, EMBL, Heidelberg, Germany.

出版信息

Yeast. 1993 Dec;9(12):1343-8. doi: 10.1002/yea.320091208.

DOI:10.1002/yea.320091208
PMID:8154185
Abstract

We have sequenced two segments containing a total of 51.6 kb of the left arm from chromosome XI of Saccharomyces cerevisiae. The first segment of 38.5 kb contains 18 open reading frames (ORFs) of more than 100 amino acid residues. Five ORFs encode known yeast genes, including the fatty acid synthase gene (FAS1). Three new yeast genes were discovered with homologies to non-yeast genes and ten new genes without homologies to any known sequences. The second segment of 13 kb contains five ORFs with two known yeast genes and three unknown genes. The sequences from cosmid pUKG041 were obtained entirely with the walking primer strategy resulting in a very low overall sequence redundancy of 2.8 and an average reading length of 443 bases.

摘要

我们对酿酒酵母第十一条染色体左臂上的两个片段进行了测序，这两个片段总长51.6 kb。第一个片段长38.5 kb，包含18个编码超过100个氨基酸残基的开放阅读框（ORF）。其中五个ORF编码已知的酵母基因，包括脂肪酸合酶基因（FAS1）。发现了三个与非酵母基因具有同源性的新酵母基因以及十个与任何已知序列均无同源性的新基因。第二个片段长13 kb，包含五个ORF，其中有两个已知的酵母基因和三个未知基因。黏粒pUKG041的序列完全通过步移引物策略获得，总体序列冗余度非常低，仅为2.8，平均读长为443个碱基。

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Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene.对酿酒酵母XI号染色体左臂上51.6千碱基的序列进行测序和分析，发现了23个开放阅读框，其中包括FAS1基因。
Yeast. 1993 Dec;9(12):1343-8. doi: 10.1002/yea.320091208.
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