Doldi N, Bassan M, Bonzi V, Ferrari A
Department of Obstetrics and Gynecology, University of Milan, San Raffaele Scientific Institute, Italy.
Gynecol Endocrinol. 1996 Apr;10(2):101-8. doi: 10.3109/09513599609097899.
To assess the direct effect of growth hormone and growth hormone-releasing hormone on gene expression of steroidogenic enzymes and production of progesterone, 17-hydroxyprogesterone (17-OHP) and estradiol, we cultured luteinizing granulosa cells with or without follicle-stimulating hormone (FSH), growth hormone and growth hormone-releasing hormone at different concentrations. Luteinizing granulosa cells were obtained from women undergoing an in vitro fertilization program in the Department of Obstetrics and Gynecology, S. Raffaele Scientific Institute, Milan, Italy. At a concentration of 1 microgram/ml, FSH significantly increased estradiol production (2.1 +/- 0.7-fold the control value; p < 0.05 vs. control) and progesterone production (3.5 +/- 2.0-fold the control value; p < 0.05 vs. control). Growth hormone was effective on estradiol, progesterone and 17-OHP at 1 microgram/ml, enhancing estradiol production (1.3 +/- 0.2-fold the control value; p < 0.05 vs. control), progesterone production (2.5 +/- 1.0-fold the control value; p < 0.05 vs. control), and 17-OHP (1.4 +/- 0.2-fold the control value; p < 0.05 vs. control). Growth hormone-releasing hormone increased estradiol production (1.5 +/- 1.2-fold the control value) and progesterone production (1.3 +/- 0.8-fold the control value), but not significantly. No effects by growth hormone-releasing hormone were seen on 17-OHP production. FSH, growth hormone and growth hormone-releasing hormone did not increase P450scc and P450 aromatase mRNAs, whereas FSH increased P450c17 mRNA to 150% at 100 ng/ml and 1 microgram/ml, growth hormone increased it to 230% at 100 ng/ml and to 200% at 1 microgram/ml, and growth hormone-releasing hormone increased it to 140% at 100 ng/ml and to 190% of control values at 1 microgram/ml. These results indicate a direct effect of growth hormone on steroidogenesis by increasing P450c17 mRNA accumulation and progesterone, 17-OHP and estradiol production.
为评估生长激素和生长激素释放激素对类固醇生成酶基因表达以及孕酮、17-羟孕酮（17-OHP）和雌二醇生成的直接作用，我们在添加或不添加不同浓度促卵泡激素（FSH）、生长激素和生长激素释放激素的情况下培养黄体化颗粒细胞。黄体化颗粒细胞取自意大利米兰圣拉斐尔科学研究所妇产科正在接受体外受精程序的女性。在浓度为1微克/毫升时，FSH显著增加了雌二醇的生成（为对照值的2.1±0.7倍；与对照相比，p<0.05）以及孕酮的生成（为对照值的3.5±2.0倍；与对照相比，p<0.05）。生长激素在1微克/毫升时对雌二醇、孕酮和17-OHP有效，提高了雌二醇的生成（为对照值的1.3±0.2倍；与对照相比，p<0.05）、孕酮的生成（为对照值的2.5±1.0倍；与对照相比，p<0.05）以及17-OHP（为对照值的1.4±0.2倍；与对照相比，p<0.05）。生长激素释放激素增加了雌二醇的生成（为对照值的1.5±1.2倍）和孕酮的生成（为对照值的1.3±0.8倍），但不显著。未观察到生长激素释放激素对17-OHP生成有影响。FSH、生长激素和生长激素释放激素均未增加P450scc和P450芳香化酶的mRNA水平，而FSH在100纳克/毫升和1微克/毫升时将P450c17 mRNA增加至150%，生长激素在100纳克/毫升时将其增加至230%，在1微克/毫升时增加至200%，生长激素释放激素在100纳克/毫升时将其增加至140%，在1微克/毫升时增加至对照值的190%。这些结果表明生长激素通过增加P450c17 mRNA积累以及孕酮、17-OHP和雌二醇的生成，对类固醇生成有直接作用。
