生长激素对从小窦卵泡中获取的人卵母细胞生发泡体外成熟的影响。
Effects of growth hormone on in vitro maturation of germinal vesicle of human oocytes retrieved from small antral follicles.
作者信息
Hassan H A, Azab H, Rahman A A, Nafee T M
机构信息
Obstetrics & Gynecology Department, Faculty of Medicine, Alexandria University, Alexandria IVF & ICSI Center, El Hedaya, Alexandria, Egypt.
出版信息
J Assist Reprod Genet. 2001 Aug;18(8):417-20. doi: 10.1023/a:1016630603560.
Abstract
PURPOSE
Evaluation of the effect of GH, administered in vivo, on in vitro maturation of human GV oocytes obtained from small follicles.
METHODS
The study included 88 ICSI women stimulated with long agonist protocol. They were randomly allocated to two groups (Group 1, 44 women with 86 GV oocytes), were cotreated with GH (Group 2, no GH: 44 women with 67 GV oocytes). GV oocytes from small follicles were matured in vitro, injected, and their development observed.
RESULTS
Resumption of meiosis was significantly higher in Group 1 compared to Group 2 (60 and 37.3% respectively). Significantly more M2 oocytes were obtained in Group 1 than in Group 2 (45.5 and 23.9% respectively). Fertilization rates were also significantly higher (51.9 and 18.8% respectively). No embryos were obtained in Group 2, whereas 21.1% of fertilized oocytes in Group 1 cleaved into class A embryos.
CONCLUSION
In vivo administration of GH enhanced in vitro maturation and fertilization of human GV oocytes retrieved from small antral follicles.
摘要
目的
评估体内注射生长激素（GH）对从小卵泡获取的人GV期卵母细胞体外成熟的影响。
方法
该研究纳入了88例采用长效激动剂方案进行刺激的ICSI女性。她们被随机分为两组（第1组，44例女性，有86个GV期卵母细胞），一组接受GH联合治疗（第2组，未用GH：44例女性，有67个GV期卵母细胞）。从小卵泡获取的GV期卵母细胞进行体外成熟、注射，并观察其发育情况。
结果
与第2组相比，第1组减数分裂的恢复率显著更高（分别为60%和37.3%）。第1组获得的M2期卵母细胞明显多于第2组（分别为45.5%和23.9%）。受精率也显著更高（分别为51.9%和18.8%）。第2组未获得胚胎，而第1组21.1%的受精卵分裂为A级胚胎。
结论
体内注射GH可增强从小窦卵泡获取的人GV期卵母细胞的体外成熟和受精。
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