Lim S H, Chew F T, Sim S M, Huang Y T, Goh D Y, Tan H T, Tan T K, Lee B W
Department of Paediatrics, National University of Singapore, Singapore.
Asian Pac J Allergy Immunol. 1995 Dec;13(2):101-5.
Skin prick tests done previously revealed a significantly higher percentage of sensitization to an extract of Bipolaris sp. among atopic individuals (34/147, 23.1%) compared to non-atopic individuals. Bipolaris-specific IgE levels were quantified in sera from a representative group of 38 individuals using the Fluorescence Allergosorbent Test (FAST). Result obtained by FAST were found to be comparable to the skin prick test results (r2 = 0.60, p < 0.001 for IgE levels vs wheal sizes; r2 = 0.44, p < 0.001 for IgE levels vs erythema sizes). Characterisation of the extract's allergenic component by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed 28 protein bands with molecular weights (MW) ranging from 11 kDa to above 100 kDa. Immunoblotting with sera of 10 Bipolaris-sensitive (skin prick test, 3 +) individuals showed that Bipolaris spore extract contained at least 4 IgE binding proteins (MW 11-13 kDa, 16-17 kDa, 20-22 kDa and 36 kDa). All 10 sera reacted to the protein at MW 20-22 kDa, 2 sera with MW 11-13 kDa, 3 sera with 16-17 kDa and 6 sera with 36 kDa. This study has thus demonstrated that spores of Bipolaris sp. contain allergenic components which may elicit IgE-mediated reactions.
先前进行的皮肤点刺试验显示，与非特应性个体相比，特应性个体中对双极霉提取物致敏的比例显著更高（34/147，23.1%）。使用荧光变应原吸附试验（FAST）对一组38名个体的血清中的双极霉特异性IgE水平进行了定量。发现FAST获得的结果与皮肤点刺试验结果具有可比性（IgE水平与风团大小的r2 = 0.60，p < 0.001；IgE水平与红斑大小的r2 = 0.44，p < 0.001）。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对提取物的变应原成分进行表征，显示出28条蛋白带，分子量（MW）范围从11 kDa到100 kDa以上。用10名对双极霉敏感（皮肤点刺试验，3 +）个体的血清进行免疫印迹分析表明，双极霉孢子提取物含有至少4种IgE结合蛋白（分子量11 - 13 kDa、16 - 17 kDa、20 - 22 kDa和36 kDa）。所有10份血清都与分子量20 - 22 kDa的蛋白发生反应，2份血清与分子量11 - 13 kDa的蛋白反应，3份血清与16 - 17 kDa的蛋白反应，6份血清与36 kDa的蛋白反应。因此，本研究表明双极霉的孢子含有可能引发IgE介导反应的变应原成分。
