Pena J M, Rubira N, Botey J, Rodrigo M J, Alonso R, Eseverri J L, Marín A, Ras R M
Biochemical, Allergy Department, Voll d'Hebron Maternity-Children's University Hospital.
Allerg Immunol (Paris). 1996 Feb;28(2):39-43.
Eosinophil Cationic Protein (ECP) is a basic protein found in eosinophil granules. This cell and its mediators are currently considered to be potential indicators of the severity of inflammation in the organism. ECP concentration can be reliably tested using several RIA or ELISA methods. It is well known that the conditions of sample obtention can affect the ECP values in blood. The aim of this study is to establish which parameters affect ECP testing during regular blood sample collection and how they affect it. Blood samples taken for the routine study of five children attended in our department were analysed: four were asthmatic and one child had atopic dermatitis. In the results we observed that ECP was not detected in the blood samples taken with EDTA tripotassium. In both the plasma samples taken with heparin as well as with serum, more ECP was released at a higher temperature. In the release of ECP obtained by coagulation, samples at 37 degrees showed values of between 4 and 20 higher than those obtained for an hour at 0 degrees. There is a considerable variability in the testing of ECP depending on the blood test extraction conditions, the range is bigger in the samples with eosinophils. These results imply the need to define a stricter protocol for obtaining samples than that suggested at present.
嗜酸性粒细胞阳离子蛋白（ECP）是一种存在于嗜酸性粒细胞颗粒中的碱性蛋白。这种细胞及其介质目前被认为是机体炎症严重程度的潜在指标。ECP浓度可以使用几种放射免疫分析（RIA）或酶联免疫吸附测定（ELISA）方法进行可靠检测。众所周知，样本采集条件会影响血液中的ECP值。本研究的目的是确定在常规血液样本采集过程中哪些参数会影响ECP检测以及它们是如何影响的。对在我们科室就诊的五名儿童的常规研究采集的血液样本进行了分析：四名患有哮喘，一名患有特应性皮炎。在结果中我们观察到，用乙二胺四乙酸三钾采集的血液样本中未检测到ECP。在使用肝素采集的血浆样本以及血清样本中，在较高温度下会释放出更多的ECP。在通过凝血获得的ECP释放中，37摄氏度的样本显示的值比在0摄氏度下放置一小时获得的值高4至20。根据血液检测提取条件，ECP检测存在相当大的可变性，嗜酸性粒细胞样本中的范围更大。这些结果意味着需要定义一个比目前建议的更严格的样本采集方案。
