Kozik A, Matvienko M, Scheres B, Paruvangada V G, Bisseling T, van Kammen A, Ellis T H, LaRue T, Weeden N
Department of Molecular Biology, Agricultural University, Wageningen, Netherlands.
Plant Mol Biol. 1996 Apr;31(1):149-56. doi: 10.1007/BF00020614.
The early nodulin gene, PsENOD7, is expressed in pea root nodules induced by Rhizobium leguminosarum bv. viciae, but not in other plant organs. In situ hybridization showed that this gene is transcribed during nodule maturation in the infected cells of the proximal part of the prefixation zone II. At the transition of zone II into interzone II-III, the level of PsENOD7 mRNA drops markedly. PsENOD7 has no significant homology to other genes. RFLP mapping studies have shown that PsENOD7 is located in linkage group I between the leghaemoglobin genes and sym2.
早期结瘤素基因PsENOD7在豌豆根瘤中表达,该根瘤由豌豆根瘤菌蚕豆生物型诱导形成,但在其他植物器官中不表达。原位杂交显示,该基因在前固定区II近端的受感染细胞的根瘤成熟过程中转录。在区域II向区域II-III过渡时,PsENOD7 mRNA水平显著下降。PsENOD7与其他基因没有显著同源性。限制性片段长度多态性图谱研究表明,PsENOD7位于血红蛋白基因和sym2之间的连锁群I中。