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豌豆凝集素插入磷脂单分子层。

Insertion of pea lectin into a phospholipid monolayer.

作者信息

Booij P, Demel R A, de Pater B S, Kijne J W

机构信息

Institute of Molecular Plant Sciences, Leiden University, Netherlands.

出版信息

Plant Mol Biol. 1996 Apr;31(1):169-73. doi: 10.1007/BF00020617.

DOI:10.1007/BF00020617
PMID:8704151
Abstract

Pea lectin (PSL) is a secretory sugar-binding protein, readily soluble in aqueous solutions of low osmolarity. However, PSL also appears to be associated with the plasma membrane at the tip of young pea root hairs. By using the Wilhelmy plate method, we found that PSL can insert into a lipid monolayer. This property appeared to be independent of the sugar-binding ability of the protein. This result suggests that PSL may be directly involved in membrane-mediated interactions with saccharide ligands, for example during root hair infection by symbiotic rhizobia.

摘要

豌豆凝集素(PSL)是一种分泌型糖结合蛋白,易溶于低渗透压的水溶液。然而,PSL似乎也与豌豆幼根毛尖端的质膜有关。通过使用威尔海姆平板法,我们发现PSL可以插入脂质单分子层。这种特性似乎与该蛋白的糖结合能力无关。这一结果表明,PSL可能直接参与与糖类配体的膜介导相互作用,例如在共生根瘤菌感染根毛的过程中。

相似文献

1
Insertion of pea lectin into a phospholipid monolayer.豌豆凝集素插入磷脂单分子层。
Plant Mol Biol. 1996 Apr;31(1):169-73. doi: 10.1007/BF00020617.
2
Mutational analysis of the sugar-binding site of pea lectin.豌豆凝集素糖结合位点的突变分析
Glycoconj J. 1994 Aug;11(4):375-80. doi: 10.1007/BF00731212.
3
Heterologous rhizobial lipochitin oligosaccharides and chitin oligomers induce cortical cell divisions in red clover roots, transformed with the pea lectin gene.异源根瘤菌脂壳寡糖和壳寡糖可诱导用豌豆凝集素基因转化的红三叶草根中的皮层细胞分裂。
Mol Plant Microbe Interact. 2000 Mar;13(3):268-76. doi: 10.1094/MPMI.2000.13.3.268.
4
[Effects of natural and hybrid lectins on the legume-rhizobium interactions].[天然和杂合凝集素对豆科植物 - 根瘤菌相互作用的影响]
Prikl Biokhim Mikrobiol. 2009 Jan-Feb;45(1):84-91.
5
Pea (Pisum sativum L.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product.豌豆(Pisum sativum L.)种子异凝集素1和2以及豌豆根凝集素是由单一基因产物经类羧肽酶加工产生的。
Plant Mol Biol. 1994 Jan;24(1):75-81. doi: 10.1007/BF00040575.
6
Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots.白三叶草毛状根中表达的豌豆凝集素的糖结合活性。
Plant Physiol. 1995 Dec;109(4):1167-1177. doi: 10.1104/pp.109.4.1167.
7
Lectin-enhanced accumulation of manganese-limited Rhizobium leguminosarum cells on pea root hair tips.凝集素增强锰限制型豌豆根瘤菌细胞在豌豆根毛尖端的积累。
J Bacteriol. 1988 Jul;170(7):2994-3000. doi: 10.1128/jb.170.7.2994-3000.1988.
8
Binding of isolated plant lectin by rhizobia during episodes of reduced gravity obtained by parabolic flight.在抛物线飞行获得的微重力条件下,根瘤菌对分离出的植物凝集素的结合。
Plant Physiol. 1990;92(1):262-4. doi: 10.1104/pp.92.1.262.
9
Destabilization of pea lectin by substitution of a single amino acid in a surface loop.豌豆凝集素表面环中单个氨基酸的取代导致其稳定性丧失。
Plant Mol Biol. 1993 Sep;22(6):1039-46. doi: 10.1007/BF00028976.
10
Mutational analysis of pea lectin. Substitution of Asn125 for Asp in the monosaccharide-binding site eliminates mannose/glucose-binding activity.豌豆凝集素的突变分析。单糖结合位点中天冬酰胺125被天冬氨酸取代会消除甘露糖/葡萄糖结合活性。
Plant Mol Biol. 1992 Dec;20(6):1049-58. doi: 10.1007/BF00028892.

引用本文的文献

1
Plant lectins: occurrence, biochemistry, functions and applications.植物凝集素:存在、生物化学、功能及应用
Glycoconj J. 2001 Aug;18(8):589-613. doi: 10.1023/a:1020687518999.
2
Root Lectins and Rhizobia.根凝集素与根瘤菌
Plant Physiol. 1997 Nov;115(3):869-873. doi: 10.1104/pp.115.3.869.
3
Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins.拟南芥lecRK-a基因的特征:一个超家族的成员,编码具有与豆科植物凝集素同源的细胞外结构域的假定受体。

本文引用的文献

1
The immuno-histochemical localization of lectin in pea seeds (Pisum sativum L.).豌豆种子(Pisum sativum L.)中凝集素的免疫组织化学定位。
Planta. 1981 Dec;153(4):287-96. doi: 10.1007/BF00384244.
2
Correlation between infection by Rhizobium leguminosarum and lectin on the surface of Pisum sativum L. roots.豌豆根表面的菜豆根瘤菌与凝集素的感染相关性。
Planta. 1986 Sep;168(3):350-9. doi: 10.1007/BF00392360.
3
Distribution of glucose/mannose-specific isolectins in pea (Pisum sativum L.) seedlings.豌豆(Pisum sativum L.)幼苗中葡萄糖/甘露糖特异性同工凝集素的分布。
Plant Mol Biol. 1999 Mar;39(4):671-82. doi: 10.1023/a:1006136701595.
Planta. 1990 Jul;181(4):451-61. doi: 10.1007/BF00192997.
4
Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots.白三叶草毛状根中表达的豌豆凝集素的糖结合活性。
Plant Physiol. 1995 Dec;109(4):1167-1177. doi: 10.1104/pp.109.4.1167.
5
A putative novel class of animal lectins in the secretory pathway homologous to leguminous lectins.分泌途径中一类与豆科凝集素同源的假定新型动物凝集素。
Cell. 1994 Jun 3;77(5):625-6. doi: 10.1016/0092-8674(94)90047-7.
6
Pea (Pisum sativum L.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product.豌豆(Pisum sativum L.)种子异凝集素1和2以及豌豆根凝集素是由单一基因产物经类羧肽酶加工产生的。
Plant Mol Biol. 1994 Jan;24(1):75-81. doi: 10.1007/BF00040575.
7
Monomolecular layers in the study of biomembranes.生物膜研究中的单分子层
Subcell Biochem. 1994;23:83-120. doi: 10.1007/978-1-4615-1863-1_3.
8
Crosslinking of mammalian lectin (galectin-1) by complex biantennary saccharides.复合双天线糖类对哺乳动物凝集素(半乳糖凝集素-1)的交联作用。
Nat Struct Biol. 1994 Dec;1(12):863-70. doi: 10.1038/nsb1294-863.
9
Monolayers--description of use and interaction.单层膜——使用及相互作用的描述。
Methods Enzymol. 1974;32:539-44. doi: 10.1016/0076-6879(74)32052-6.
10
The crystal structure of pea lectin at 3.0-A resolution.豌豆凝集素在3.0埃分辨率下的晶体结构。
J Biol Chem. 1986 Dec 15;261(35):16518-27.