Hanano S, Sugita M, Sugiura M
Center for Gene Research, Nagoya University, Japan.
Plant Mol Biol. 1996 Apr;31(1):57-68. doi: 10.1007/BF00020606.
A cDNA encoding a protein with a consensus sequence-type RNA-binding domain (CS-RBD) has been isolated from a Nicotiana sylvestris cDNA library. The deduced protein (designated 'RZ-1') contains CS-RBD in its N-terminal half, arginine/aspartic acid repeats in its center and a glycine-rich-C-terminal region in which a zinc finger motif of the CCHC type is present. The corresponding gene appears to be expressed constitutively in all tobacco organs. Immunocytochemical assays revealed that RZ-1 is localized in the nucleoplasm of tobacco cultured cells. Glycerol gradient fractionation of tobacco nuclear lysates showed that RZ-1 is associated with a large ribonucleoprotein particle of around 60 S in size. Nucleic acid-binding assays indicated that RZ-1 binds preferentially to poly (G) and both the CS-RBD and glycine-rich region are necessary for its binding activity. A possible role of RZ-1 is discussed.
已从野生烟草cDNA文库中分离出一个编码具有共有序列型RNA结合结构域（CS-RBD）的蛋白质的cDNA。推导的蛋白质（命名为“RZ-1”）在其N端一半含有CS-RBD，在其中心含有精氨酸/天冬氨酸重复序列，以及在其富含甘氨酸的C端区域存在一个CCHC型锌指基序。相应的基因似乎在所有烟草器官中组成型表达。免疫细胞化学分析表明，RZ-1定位于烟草培养细胞的核质中。烟草核裂解物的甘油梯度分级分离表明，RZ-1与大小约为60S的大核糖核蛋白颗粒相关。核酸结合分析表明，RZ-1优先结合聚（G），并且CS-RBD和富含甘氨酸的区域对其结合活性都是必需的。文中讨论了RZ-1的可能作用。
