气相色谱-负离子化学电离质谱法分析毛发中的去甲西泮和奥沙西泮

Hair analysis for nordiazepam and oxazepam by gas chromatography--negative-ion chemical ionization mass spectrometry.

作者信息

Kintz P, Cirimele V, Vayssette F, Mangin P

机构信息

Institut de Médecine Légale, Strasbourg, France.

出版信息

J Chromatogr B Biomed Appl. 1996 Mar 3;677(2):241-4. doi: 10.1016/0378-4347(95)00444-0.

DOI:10.1016/0378-4347(95)00444-0

PMID:8704927

Abstract

A procedure is presented for the identification of nordiazepam and its metabolite, oxazepam, in human hair. The method involves decontamination of hair with dichloromethane, incubation in phosphate buffer (pH 7.6) in the presence of deuterated internal standards, liquid-liquid extraction, derivatization with N,O-bis(trimethylsilyl)trifluoroacetamide plus 1% trimethylchlorosilane and gas chromatography-mass spectrometry using negative-ion chemical ionization with methane. Among thirty samples obtained from polydrug abusers, thirteen tested positive for nordiazepam, in the range of 0.25-18.87 ng/mg. Five samples were also positive for oxazepam, in the range 0.11-0.50 ng/mg.

摘要

本文介绍了一种用于鉴定人发中去甲西泮及其代谢物奥沙西泮的方法。该方法包括用二氯甲烷对头发进行去污处理，在氘代内标存在的情况下于磷酸盐缓冲液（pH 7.6）中孵育，液液萃取，用N,O-双（三甲基硅基）三氟乙酰胺加1%三甲基氯硅烷进行衍生化，以及使用甲烷负离子化学电离的气相色谱-质谱联用。在从多药滥用者处获得的30个样本中，13个样本检测出含有去甲西泮，含量范围为0.25 - 18.87 ng/mg。5个样本还检测出含有奥沙西泮，含量范围为0.11 - 0.50 ng/mg。