Vayssette F, Cirimele V, Kintz P, Mangin P
Institut de Médecine Légale, Strasbourg.
Ann Pharm Fr. 1996;54(5):211-6.
Hair samples were obtained from 36 drug addicts deceased from fatal heroin overdose. Samples were decontaminated with dichloromethane and pulverized. 50 mg of powdered hair were incubated 2 h at 40 degrees C in a phosphate buffer pH 7.6 in presence of deuterated internal standards (Nordiazepam-d5 and Oxazepam-d5) and extracted by a mixture ether/chloroform (80:20, v/v) at pH 8.4. After evaporation of the solvent, the dry extract was derivatized by silylation (BSTFA + 1% TMCS) and analyzed by gas chromatography coupled to mass spectrometry, with a detector operating in negative chemical ionization mode. 13 samples tested positive for Nordiazepam with concentrations ranging from 0.25 to 18.87 ng/mg of hair (mean: 4.16 ng/mg) and 5 positives for its metabolite, Oxazepam, with concentrations ranging from 0.11 to 0.50 ng/mg of hair (mean : 0.28 ng/mg).
从36名死于致命海洛因过量的吸毒者身上获取毛发样本。样本用二氯甲烷进行净化处理并粉碎。取50毫克毛发粉末，在含氘代内标（去甲西泮-d5和奥沙西泮-d5）的pH 7.6磷酸盐缓冲液中于40℃孵育2小时，然后在pH 8.4条件下用乙醚/氯仿混合液（80:20，v/v）萃取。溶剂蒸发后，干燥提取物通过硅烷化（BSTFA + 1% TMCS）进行衍生化处理，并采用与质谱联用的气相色谱进行分析，检测器以负化学电离模式运行。13个样本去甲西泮检测呈阳性，毛发浓度范围为0.25至18.87纳克/毫克（均值：4.16纳克/毫克）；其代谢产物奥沙西泮有5个阳性样本，毛发浓度范围为0.11至0.50纳克/毫克（均值：0.28纳克/毫克）。
