Kapuściński A
Department of Neuropathology, Medical Research Centre, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1995;33(2):101-4.
In 47 adult rats 10-min cardiac arrest was induced by the intrathoracic compression of the heart vessel bundle. The animals were sacrificed at 15, 30, 60, 120 min and 6 h or 1, 3, 7 days after resuscitation. Decapitation was performed 15 sec after intracarotid injection of mixture of L-[U-14C] phenylalanine (PHE) and tritiated water in PBS buffer. By the dual label method the brain uptake index (BUI) and percent of injected dose of amino acid in the cerebral hemisphere were calculated. A decrease of PHE uptake and drop of BUI revealed the blood-brain barrier (BBB) alterations resulting in diminution of amino acid transport into brain. The most pronounced changes developed between 15 and 120 min after resuscitation and also after 7 days. The above data revealed the decreased active transport of PHE in the early and late periods after ischemic insult.
在47只成年大鼠中，通过胸腔内压迫心脏血管束诱导10分钟的心脏骤停。在复苏后15分钟、30分钟、60分钟、120分钟以及6小时或1天、3天、7天处死动物。在颈内动脉注射溶解于PBS缓冲液中的L-[U-¹⁴C]苯丙氨酸（PHE）和氚水的混合物15秒后进行断头。通过双标记法计算脑摄取指数（BUI）和大脑半球中氨基酸注射剂量的百分比。PHE摄取的减少和BUI的下降揭示了血脑屏障（BBB）的改变，导致氨基酸向脑内的转运减少。最明显的变化发生在复苏后15至120分钟之间以及7天后。上述数据揭示了缺血性损伤后早期和晚期PHE主动转运的减少。
