Kapuściński A, Nikolaishvili L
Department of Neuropathology, Medical Research Centre, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1996;34(2):72-5.
In adult rats 10-min cardiac arrest was induced by the intracarotid compression of the heart vessel bundle. The animals were sacrificed at 2 weeks and 1, 2, 3 months after resuscitation. Decapitation was performed 15 sec after intracarotid injection of mixture of L-[methyl-3H]methionine (MET) and 4-iodo[n-methyl-14C]antipyrine. By means of the dual label method the brain uptake index (BUI) of amino acid (AA) in the cerebral hemisphere was calculated. MET BUI was corrected for residual vascular radioactivity after injection of [14C]sucrose. The significant increase of MET BUI was observed 1 and 2 months after resuscitation, reaching approximately 2-fold of control values. Two weeks and 3 months after resuscitation values did not differ significantly from control. The present study revealed either periodical increase of BBB permeability for MET or increased transport of AA according to metabolic needs during the postischemic reparative processes.
在成年大鼠中，通过颈内动脉压迫心脏血管束诱导10分钟心脏骤停。在复苏后2周以及1、2、3个月处死动物。在颈内动脉注射L-[甲基-³H]蛋氨酸（MET）和4-碘-[N-甲基-¹⁴C]安替比林的混合物15秒后进行断头。通过双标记法计算大脑半球中氨基酸（AA）的脑摄取指数（BUI）。注射[¹⁴C]蔗糖后，对MET BUI进行残余血管放射性校正。在复苏后1个月和2个月观察到MET BUI显著增加，达到对照值的约2倍。复苏后2周和3个月的值与对照无显著差异。本研究揭示了在缺血后修复过程中，要么BBB对MET的通透性呈周期性增加，要么根据代谢需求氨基酸转运增加。
