Deshmukh S R, Mantri S B, Kendre P B, Nagoba B S
Department of Microbiology, M.I.M.S.R. Medical College, Latur.
Indian J Med Res. 1996 Jun;103:294-5.
A comparative study of Ziehl-Neelsen stain, cold stain and modified Schaeffer and Fulton stain was carried out to evaluate the efficiency of modified Schaeffer and Fulton method in sputum examination for acid fast bacilli (AFB). Of 187 sputum samples studied, 67 (35.82%) were reported positive by Ziehl-Neelsen stain and cold stain method while 66 (35.29%) were reported positive by modified Schaeffer and Fulton method. In comparison with Ziehl-Neelsen staining, 98.58 per cent positivity was reported by modified Schaeffer and Fulton method. Modified Schaeffer and Fulton method is found to be simple, reliable, less expensive and as efficient as Ziehl-Neelsen stain and cold stain for demonstration of acid fast bacilli in sputum.
开展了齐-尼氏染色、冷染色和改良沙-富氏染色的对比研究,以评估改良沙-富氏方法在痰液抗酸杆菌(AFB)检测中的效率。在研究的187份痰液样本中,67份(35.82%)通过齐-尼氏染色和冷染色方法报告为阳性,而66份(35.29%)通过改良沙-富氏方法报告为阳性。与齐-尼氏染色相比,改良沙-富氏方法的阳性率为98.58%。结果发现,改良沙-富氏方法简单、可靠、成本较低,并且在痰液中显示抗酸杆菌方面与齐-尼氏染色和冷染色一样有效。