Tillman W, Labitxke N, Schröter W
Klin Wochenschr. 1977 Apr 15;55(8):385-91. doi: 10.1007/BF01488624.
The rheological properties of erythrocytes of 6 patients with glucose-6-phosphate dehydrogenase deficiency were studied. A method of filtration and measurements of viscosity of erythrocyte suspensions with a hematocrit of 80% were used. The erythrocytes of all patients were more flexible than the erythrocytes of healthy controls. The erythrocytes of the patients passed the polycarbonate filters with a pore diameter of 5 micron more rapidly and showed a lower viscosity of erythrocyte suspensions. The increased flexibility of erythrocytes was due to a lower hemoglobin content which was measured in isolated ghosts. After incubation of the cells with acetylphenylhydrazine, Heinz bodies formed in the erythrocytes of controls. The flexibility of these cells decreased markedly. Though more Heinz bodies were found in the erythrocytes of the patients when the cells were incubated under identical conditions, even these erythrocytes became less rigid than the erythrocytes of controls. From these results we condlude that the more flexible erythrocytes of the patients could pass the splenic sinus even when they contain Heinz bodies. The rheological properties of the erythrocytes explain why splenectomy is not efficient in glucose-6-phosphate dehydrogenase deficiency.
对6例葡萄糖-6-磷酸脱氢酶缺乏症患者的红细胞流变学特性进行了研究。采用了一种过滤方法，并对血细胞比容为80%的红细胞悬液的粘度进行了测量。所有患者的红细胞比健康对照者的红细胞更具柔韧性。患者的红细胞能更快地通过孔径为5微米的聚碳酸酯滤器，且红细胞悬液的粘度较低。红细胞柔韧性增加是由于在分离的空壳中测得的血红蛋白含量较低。用乙酰苯肼孵育细胞后，对照组红细胞中形成了海因茨小体。这些细胞的柔韧性明显降低。尽管在相同条件下孵育时，患者红细胞中发现了更多的海因茨小体，但即使是这些红细胞也比对照组红细胞的刚性小。从这些结果我们得出结论，患者红细胞更具柔韧性，即使含有海因茨小体也能通过脾窦。红细胞的流变学特性解释了为什么脾切除术对葡萄糖-6-磷酸脱氢酶缺乏症无效。
