Tawfik A F, Al-Zamil F A, Ramadan M A, Shibl A M
Department of Clinical Laboratory Sciences, King Saud University, Riyadh, Saudi Arabia.
J Chemother. 1996 Apr;8(2):102-6. doi: 10.1179/joc.1996.8.2.102.
The effects of the beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam on normal immune responses were investigated. These agents did not interfere with either humoral or cell-mediated immune responses as measured by the hemolytic plaque assay and delayed type hypersensitivity reaction assay respectively. In addition, human polymorphonuclear leukocyte phagocytic activity was not altered by these agents. When these agents were tested for their effect on Staphylococcus aureas adherence to buccal epithelial cells we found that all inhibitors suppressed staphylococcal adherence at therapeutic serum concentrations. Among the inhibitors investigated, sulbactam was found to significantly inhibit the hemolysin production of S. aureus. These data suggest that beta-lactamase inhibitors do not exhibit immunomodulating activity, but they interfere with some of the virulence factors of S. aureus. These findings suggest the advantage of preparations containing these inhibitors.
研究了β-内酰胺酶抑制剂克拉维酸、舒巴坦和他唑巴坦对正常免疫反应的影响。通过溶血空斑试验和迟发型超敏反应试验分别测定，这些药物对体液免疫或细胞介导的免疫反应均无干扰。此外，这些药物不会改变人多形核白细胞的吞噬活性。当测试这些药物对金黄色葡萄球菌黏附颊黏膜上皮细胞的影响时，我们发现所有抑制剂在治疗性血清浓度下均能抑制葡萄球菌的黏附。在所研究的抑制剂中，发现舒巴坦能显著抑制金黄色葡萄球菌溶血素的产生。这些数据表明，β-内酰胺酶抑制剂不具有免疫调节活性，但它们会干扰金黄色葡萄球菌的一些毒力因子。这些发现提示了含有这些抑制剂的制剂的优势。
