原发性人类免疫缺陷病毒的中和抗性并不需要增加包膜刺突密度和稳定性。
Increased envelope spike density and stability are not required for the neutralization resistance of primary human immunodeficiency viruses.
作者信息
Karlsson G B, Gao F, Robinson J, Hahn B, Sodroski J
机构信息
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
出版信息
J Virol. 1996 Sep;70(9):6136-42. doi: 10.1128/JVI.70.9.6136-6142.1996.
Abstract
Previous observations that the gp120 envelope glycoprotein contents of some primary, clade B human immunodeficiency virus type 1 (HIV-1) isolates were higher than those of laboratory-passaged HIV-1 isolates suggested the hypothesis that increased envelope glycoprotein spike density or stability contributes to the relative neutralization resistance of the primary viruses. To test this, the structural, replicative, and neutralization properties of a panel of recombinant viruses with HIV-1 envelope glycoproteins from divergent clades were examined in an env complementation assay. In this system, although the spike density and stability of envelope glycoproteins from primary HIV-1 isolates were not greater than those from a laboratory-adapted isolate, relative resistance to neutralizing antibodies and soluble CD4 was observed for the viruses with primary envelope glycoproteins. Thus, neither high envelope glycoprotein spike density nor stability is necessary for the relative neutralization resistance of primary HIV-1 viruses.
摘要
先前的观察结果表明，一些B亚型人类免疫缺陷病毒1型（HIV-1）原代分离株的gp120包膜糖蛋白含量高于实验室传代的HIV-1分离株，这提示了一个假说，即包膜糖蛋白刺突密度或稳定性的增加有助于原代病毒的相对中和抗性。为了验证这一点，在env互补试验中检测了一组具有来自不同分支的HIV-1包膜糖蛋白的重组病毒的结构、复制和中和特性。在这个系统中，虽然原代HIV-1分离株的包膜糖蛋白刺突密度和稳定性并不高于实验室适应株，但具有原代包膜糖蛋白的病毒对中和抗体和可溶性CD4表现出相对抗性。因此，原代HIV-1病毒的相对中和抗性既不需要高包膜糖蛋白刺突密度，也不需要稳定性。
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