一种高度分化的非洲人类免疫缺陷病毒分离株的基因组克隆及全序列分析
Genomic cloning and complete sequence analysis of a highly divergent African human immunodeficiency virus isolate.
作者信息
Vanden Haesevelde M, Decourt J L, De Leys R J, Vanderborght B, van der Groen G, van Heuverswijn H, Saman E
机构信息
Innogenetics N.V., Gent, Belgium.
出版信息
J Virol. 1994 Mar;68(3):1586-96. doi: 10.1128/JVI.68.3.1586-1596.1994.
Abstract
Analysis of the complete sequence of a human immunodeficiency virus (HIV) isolate (Ant70) obtained from a Cameroonian patient indicates that this virus is the most divergent strain within the HIV-1 family hitherto described. Comparison of the Pol protein, usually highly conserved within the HIV-1 family, shows only about 73% similarity with the HIVmm isolate, whereas for the more variable proteins such as envelope, similarities of 50% or lower are found. The principal neutralizing determinant (V3 loop) and the immunodominant region within gp41 also contain some unusual substitutions, which may have implications for protein function as well as for serological assays based on these regions. Phylogenetic analyses show that this isolate occupies a unique position relative to the human HIV-1 isolates and the recently described SIVcpz virus, indicating that this Cameroonian isolate may provide us with new insights into the origins of the HIV-1 family.
摘要
对从一名喀麦隆患者身上分离出的人类免疫缺陷病毒（HIV）毒株（Ant70）的完整序列进行分析表明，该病毒是迄今为止所描述的HIV - 1家族中差异最大的毒株。通常在HIV - 1家族中高度保守的Pol蛋白，与HIVmm毒株相比，相似度仅约为73%，而对于诸如包膜等变异性更高的蛋白，相似度则为50%或更低。主要中和决定簇（V3环）以及gp41内的免疫显性区域也包含一些不寻常的替代，这可能对蛋白质功能以及基于这些区域的血清学检测有影响。系统发育分析表明，相对于人类HIV - 1分离株和最近描述的SIVcpz病毒，该分离株占据独特位置，这表明该喀麦隆分离株可能为我们提供关于HIV - 1家族起源的新见解。
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