Ghangas G S, Milman G
Science. 1977 Jun 3;196(4294):1119-20. doi: 10.1126/science.870972.
Immunoprecipitated hypoxanthine phosphoribosyltransferase (HPRT) from hemolyzates displays two major spots after two-dimensional polyacrylamide gel electrophoresis. HeLa cells or human lymphoblasts display only a single HPRT spot located at the same position as the most basic of the hemolyzate HPRT spots. This suggests that the most basic spot is the form initially synthesized, and the more acidic hemolyzate HPRT spot (a pseudoisozyme) is probably derived from the first by an age-related modification (for example, deamidation). The HPRT pattern of the hemolyzate from a Lesch-Nyhan patient was shifted to a more basic isoelectric pH, implying the mutation of a structural gene.
从溶血产物中免疫沉淀的次黄嘌呤磷酸核糖转移酶（HPRT）在二维聚丙烯酰胺凝胶电泳后显示出两个主要斑点。HeLa细胞或人淋巴母细胞仅显示一个HPRT斑点，其位置与溶血产物HPRT斑点中最碱性的斑点相同。这表明最碱性的斑点是最初合成的形式，而溶血产物中酸性更强的HPRT斑点（一种假同功酶）可能是由第一种形式通过与年龄相关的修饰（例如脱酰胺作用）衍生而来。莱施-奈恩病患者溶血产物的HPRT模式向更碱性的等电pH值偏移，这意味着结构基因发生了突变。
