Kamioka H, Maeda E, Jimbo Y, Robinson H P, Kawana A
Nippon Telegraph Laboratory, Kanagawa, Japan.
Neurosci Lett. 1996 Mar 15;206(2-3):109-12. doi: 10.1016/s0304-3940(96)12448-4.
Long-term recording of spontaneous activity in cultured cortical neuronal networks was carried out using substrates containing multi-electrode arrays. Spontaneous uncorrelated firing appeared within the first 3 days and transformed progressively into synchronized bursting within a week. By 30 days from the establishment of the culture, the network exhibited a complicated non-periodic, synchronized activity pattern which showed no changes for more than 2 months and thus represented the mature state of the network. Pharmacological inhibition of activity only during the period when regular synchronized bursting was observed was capable of producing a different mature activity pattern from the control. These results suggest that periodic synchronized bursting plays a critical role in the development of synaptic connections.
使用包含多电极阵列的基质对培养的皮质神经元网络中的自发活动进行长期记录。自发的非相关放电在最初3天内出现，并在一周内逐渐转变为同步爆发。从培养建立起30天时，网络呈现出复杂的非周期性同步活动模式，该模式在两个多月内没有变化，因此代表了网络的成熟状态。仅在观察到规则同步爆发的期间对活动进行药理学抑制能够产生与对照不同的成熟活动模式。这些结果表明，周期性同步爆发在突触连接的发育中起关键作用。
