通过免疫荧光在体内和体外研究鸡胚中的晶状体抗原发育。
Lens antigen development in chick embryos studied in vivo and in vitro by immunofluorescence.
作者信息
Rahmani T M
出版信息
Acta Anat (Basel). 1979;104(1):86-98.
Lens antigens, detected by immunofluorescence using rabbit antiserum against adult chick lens, appear in the chick embryo at stage 16. When eye rudiments are cultured in vitro, antigens developed; but they did not when optic cups were cultured but for a few cases. Isolated presumptive lens ectoderm from stage 4 did not develop antigens when cultured, but such ectoderm from stages 7--9 developed lens antigens and also showed lens structures. Stage 4 ectoderm could be induced to lens antigen development by alcohol-killed cups from stages 9--13. The experimental system can be used for in vitro studies on lens induction.
使用兔抗成年鸡晶状体抗血清通过免疫荧光检测到的晶状体抗原，在第16阶段出现在鸡胚中。当眼原基在体外培养时，抗原会发育；但当视杯培养时，除少数情况外，抗原不会发育。从第4阶段分离的假定晶状体外胚层在培养时不会发育抗原，但来自第7 - 9阶段的这种外胚层会发育晶状体抗原并也显示出晶状体结构。第4阶段的外胚层可以被来自第9 - 13阶段经酒精灭活的视杯诱导发育出晶状体抗原。该实验系统可用于晶状体诱导的体外研究。
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