Esposito D, Fitzmaurice W P, Benjamin R C, Goodman S D, Waldman A S, Scocca J J
Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA.
Nucleic Acids Res. 1996 Jun 15;24(12):2360-8. doi: 10.1093/nar/24.12.2360.
The complete nucleotide sequence of the temperate phage HP1 of Haemophilus influenzae was determined. The phage contains a linear, double-stranded genome of 32 355 nt with cohesive termini. Statistical methods were used to identify 41 probable protein coding segments organized into five plausible transcriptional units. Regions encoding proteins involved in recombination, replication, transcriptional control, host cell lysis and phage production were identified. The sizes of proteins in the mature HP1 particle were determined to assist in identifying genes for structural proteins. Similarities between HP1 coding sequences and those in databases, as well as similar gene organizations and control mechanisms, suggest that HP1 is a member of the P2-like phage family, with strong similarities to coliphages P2 and 186 and some similarity to the retronphage Ec67.
测定了流感嗜血杆菌温和噬菌体HP1的完整核苷酸序列。该噬菌体含有一个32355 nt的线性双链基因组,具有粘性末端。使用统计方法鉴定了41个可能的蛋白质编码片段,这些片段被组织成五个可能的转录单元。确定了编码参与重组、复制、转录控制、宿主细胞裂解和噬菌体产生的蛋白质的区域。测定了成熟HP1颗粒中蛋白质的大小,以帮助鉴定结构蛋白的基因。HP1编码序列与数据库中的序列之间的相似性,以及相似的基因组织和控制机制,表明HP1是P2样噬菌体家族的成员,与大肠杆菌噬菌体P2和186有很强的相似性,与反转录噬菌体Ec67有一些相似性。