小鼠Hoxd-3同源框基因的序列与表达

Sequence and expression of the murine Hoxd-3 homeobox gene.

作者信息

Tan D P, Shao X, Pu L, Guo V, Nirenberg M

机构信息

Laboratory of Biochemical Genetics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8247-52. doi: 10.1073/pnas.93.16.8247.

DOI:10.1073/pnas.93.16.8247

PMID:8710855

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC38655/

Abstract

Murine Hoxd-3 (Hox 4.1) genomic DNA and cDNA and Hoxa-3 (Hox 1.5) cDNA were cloned and sequenced. The homeodomains of Hoxd-3 and Hoxa-3 and regions before and after the homeodomain are highly conserved. Both Hoxa-3 and Hoxa-3 proteins have a proline-rich region that contains consensus amino acid sequences for binding to Src homology 3 domains of some signal transduction proteins. Northern blot analysis of RNA from 8- to 11-day-old mouse embryos revealed a 4.3-kb species of Hoxd-3 RNA, whereas a less abundant 3.0-kb species of Hoxd-3 RNA was found in RNA from 9- to 11-day-old embryos. Two species of Hoxd-3 poly(A)+ RNA, 4.3 and 6.0 kb in length, were found in poly(A)+ RNA from adult mouse kidney, but not in RNA from other adult tissues tested. Hoxd-3 mRNA was detected by in situ hybridization in 12-, 14-, and 17-day-old mouse embryos in the posterior half of the myelencephalon, spinal cord, dorsal root ganglia, first cervical vertebra, thyroid gland, kidney tubules, esophagus, stomach, and intestines.

摘要

克隆并测序了小鼠Hoxd - 3（Hox 4.1）基因组DNA、cDNA以及Hoxa - 3（Hox 1.5）cDNA。Hoxd - 3和Hoxa - 3的同源结构域以及同源结构域前后的区域高度保守。Hoxa - 3和Hoxa - 3蛋白都有一个富含脯氨酸的区域，其中包含与某些信号转导蛋白的Src同源3结构域结合的共有氨基酸序列。对8至11日龄小鼠胚胎的RNA进行Northern印迹分析，发现了一种4.3 kb的Hoxd - 3 RNA，而在9至11日龄胚胎的RNA中发现了一种丰度较低的3.0 kb的Hoxd - 3 RNA。在成年小鼠肾脏的多聚腺苷酸（poly(A)+）RNA中发现了两种长度分别为4.3 kb和6.0 kb的Hoxd - 3 poly(A)+ RNA，但在测试的其他成年组织的RNA中未发现。通过原位杂交在12日龄、14日龄和17日龄小鼠胚胎的延髓后半部、脊髓、背根神经节、第一颈椎、甲状腺、肾小管、食管、胃和肠道中检测到了Hoxd - 3 mRNA。