Driscoll P, Ferré P, Fernández-Teruel A, Levi de Stein M, Wolfman C, Medina J, Tobeña A, Escorihuela R M
Institut für Nutztierwissenschaften, ETH-Zentrum, Zürich, Switzerland.
Psychopharmacology (Berl). 1995 Nov;122(1):51-7. doi: 10.1007/BF02246441.
Utilizing psychogenetically selected Roman high- and low-avoidance rats (RHA/Verh and RLA/Verh), the present experiments investigated the effects of prenatally administered vehicle and diazepam (1 and 3 mg/kg per day, SC) on the behavior and neurochemistry of adult, male offspring. Active, two-way avoidance behavior was analyzed in 96 rats, at 6 months of age, and swimming navigation in 68 others, at 11 months. Three weeks after testing, selected brain areas from the latter animals were immunoassayed for benzodiazepine (BZD)-like molecules. The 3 mg/kg dose of diazepam both decreased freezing behavior in the shuttle box and reduced the hippocampal content of BZD-like molecules in the RLA/Verh male rats. Swimming navigation (spatial learning), at which the RLA/Verh rats were more adept, was not specifically affected by prenatal diazepam in either rat line. The possibility exists that an increased hippocampal release of BZD-like substances may be necessary to alter shuttle box behavior in RLA/Verh rats.
利用心理遗传学筛选出的罗马高回避和低回避大鼠（RHA/Verh和RLA/Verh），本实验研究了产前给予溶剂和地西泮（每天1和3毫克/千克，皮下注射）对成年雄性后代行为和神经化学的影响。对96只6个月大的大鼠的主动双向回避行为进行了分析，对另外68只11个月大的大鼠的游泳导航能力进行了分析。测试三周后，对后一组动物的选定脑区进行了苯二氮䓬（BZD）样分子的免疫测定。3毫克/千克剂量的地西泮既降低了穿梭箱中的僵住行为，又降低了RLA/Verh雄性大鼠海马中BZD样分子的含量。RLA/Verh大鼠更擅长的游泳导航（空间学习），在两种品系的大鼠中均未受到产前地西泮的特异性影响。有可能增加海马中BZD样物质的释放对于改变RLA/Verh大鼠的穿梭箱行为是必要的。
