Pei X T, Wu Z Z, Coutinho L H, Testa N G, Dexter T M
Beijing Institute of Radiation Medicine.
Sheng Li Xue Bao. 1995 Oct;47(5):485-90.
CD34+ cells were isolated from human umbilical cord blood by using a high-gradient magnetic cell sorting system with anti-CD34 monoclonal antibody coated with microbeads, and then inoculated onto a pre-formed irradiated bone marrow stroma to evaluate the grafting efficiency of CD34+ cells with rhGM-CSF or IL-3, alone or in combination. The results showed that only 36% CD34+ cells in the control, and 68 - 89.6% in the groups with growth factors treatment seeded to the stroma. A short incubation of CD34+ cells with growth factors could rapidly reconstitute a long-term hemopoiesis in the long-term liquid culture system. This demonstrates that a brief treatment of CD34+ cells with GM-CSF or IL-3 can improve the grafting efficiency of transplanted umbilical cord blood cells.
使用包被有抗CD34单克隆抗体微珠的高梯度磁性细胞分选系统从人脐带血中分离出CD34+细胞，然后接种到预先照射过的骨髓基质上，以评估单独或联合使用重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）或白细胞介素-3（IL-3）时CD34+细胞的植入效率。结果显示，对照组中只有36%的CD34+细胞接种到了基质上，而在生长因子处理组中这一比例为68%-89.6%。CD34+细胞与生长因子短暂孵育能够在长期液体培养系统中快速重建长期造血功能。这表明用GM-CSF或IL-3对CD34+细胞进行短暂处理可以提高移植脐带血细胞的植入效率。
