Pei Xuetao
Beijing Institute of Transfusion Medicine, China.
Int J Hematol. 2002 Aug;76 Suppl 1:155-6. doi: 10.1007/BF03165233.
Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. Over the last few years, transplantation of hematopoietic stem and progenitor cells from mobilized blood stem cells, umbilical cord blood and selected CD34+ cells has been used for treatment of patients with hematologic and non-hematologic malignances. The techniques have become available that allow the extensive proliferation, orderly differentiation, functional activation and gene transfer of hematopoietic stem/progenitor cells in ex vivo culture systems. These techniques have now developed to the point at which clinical trials are now underway in a variety of settings for the applications of hematopoietic stem cell transplantation, hematopoietic support after high-dose chemotherapy, immunotherapy of cancers, and gene therapy. The article will discuss the characteristics, detecting assays, surface markers, expansion, orderly differentiation, cell therapy and gene therapy of hematopoietic stem/progenitor cells. Except that, it will also discuss some advanced achievements of stem cell research, such as the multilineage differentiation of marrow-derived mesenchymal stem cells. Some results of different gene expression between stem cells and induced cells by gene chip analysis will be reported.
造血干细胞（HSCs）通过利用其自我更新和多谱系分化能力，终生供应所有血细胞。在过去几年中，来自动员的造血干细胞、脐带血和选定的CD34+细胞的造血干细胞和祖细胞移植已被用于治疗血液系统和非血液系统恶性肿瘤患者。现已出现了一些技术，可使造血干/祖细胞在体外培养系统中大量增殖、有序分化、功能激活和基因转移。这些技术现已发展到目前正在各种情况下进行临床试验的阶段，用于造血干细胞移植、大剂量化疗后的造血支持、癌症免疫治疗和基因治疗。本文将讨论造血干/祖细胞的特征、检测方法、表面标志物、扩增、有序分化、细胞治疗和基因治疗。除此之外，还将讨论干细胞研究的一些先进成果，如骨髓间充质干细胞的多谱系分化。将报告基因芯片分析干细胞与诱导细胞之间不同基因表达的一些结果。
