Li Y, Han C D
Institute of Vascular Medicine, Third School of Clinical Medicine, Beijing Medical University.
Sheng Li Xue Bao. 1995 Oct;47(5):498-504.
The effect of alpha 1-Adrenoceptor (alpha 1-AR) and its subtypes, alpha 1A and alpha 1B, activation on the proliferation of cultured vascular smooth muscle cells (VSMC) was studied by [3H]-thymidine (3H-TdR) incorporation and in situ hybridization. Norepinephrine (NE) promotes DNA synthesis of VSMC. The action was induced by activation of alpha 1A-AR subtype and increase of c-fos gene expression. However, the DNA synthesis was inhibited by activation of alpha 1B-AR subtype. There was a synergetic effect on VSMC DNA synthesis between NE and neuropeptide Y (NPY). But only additional effect was found between NE and Ang II. More over, it was found that alpha 1B-AR subtype showed significant constitutive inhibitory effect of VSMC DNA synthesis.
通过[3H]-胸腺嘧啶核苷（3H-TdR）掺入法和原位杂交技术，研究了α1-肾上腺素能受体（α1-AR）及其亚型α1A和α1B的激活对培养的血管平滑肌细胞（VSMC）增殖的影响。去甲肾上腺素（NE）促进VSMC的DNA合成。该作用是由α1A-AR亚型的激活和c-fos基因表达的增加所诱导的。然而，α1B-AR亚型的激活抑制了DNA合成。NE和神经肽Y（NPY）对VSMC的DNA合成有协同作用。但NE和血管紧张素II（Ang II）之间仅发现有附加作用。此外，发现α1B-AR亚型对VSMC的DNA合成具有显著的组成性抑制作用。
