在碳氢化合物上生长的解糖莫拉克斯氏菌和洛菲莫拉克斯氏菌肽聚糖的结构

Structure of the peptidoglycans of Moraxella glucidolytica and Moraxella lwoffi grown on hydrocarbons.

作者信息

Horisberger M

出版信息

Arch Microbiol. 1977 Apr 1;112(3):297-302. doi: 10.1007/BF00413096.

Abstract

The peptidoglycans of Moraxella glucidolytica and Moraxella lwoffi grown on aliphatic hydrocarbons were isolated. They contained muramic acid, glucosamine, alanine, D-glutamic acid and mesodiaminoimelic acid in a molar ratio of about 0.5:0.5:1.6:1.0:1.0 (M. glucidolytica) and 0.8:0.7:1.3:1.0:1.0 (M. lwoffi). The peptidoglycans were lysozyme-resistant. However, when treated with formanide, they could be partially degraded by lysozyme. The fragments were purified and their structure determined. In both strains, the peptide subunits consisted mainly of tripeptides (L-Ala-D-Glu-meso-DAP) and tetrapeptides (L-Ala-D-glu-meso-DAP-D-Ala), most of them being directly cross-linked. It is concluded that in both strains the primary structures of the peptidoglycans are closely related.

摘要

分离出了在脂肪烃上生长的解糖莫拉菌和洛菲莫拉菌的肽聚糖。它们含有胞壁酸、葡萄糖胺、丙氨酸、D - 谷氨酸和内消旋二氨基庚二酸，摩尔比约为0.5:0.5:1.6:1.0:1.0（解糖莫拉菌）和0.8:0.7:1.3:1.0:1.0（洛菲莫拉菌）。这些肽聚糖对溶菌酶具有抗性。然而，用甲酰胺处理后，它们可被溶菌酶部分降解。对片段进行了纯化并确定了其结构。在这两种菌株中，肽亚基主要由三肽（L - 丙氨酸 - D - 谷氨酸 - 内消旋二氨基庚二酸）和四肽（L - 丙氨酸 - D - 谷氨酸 - 内消旋二氨基庚二酸 - D - 丙氨酸）组成，其中大多数是直接交联的。得出的结论是，在这两种菌株中，肽聚糖的一级结构密切相关。