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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Knox K W, Holmwood K J

Biochem J. 1968 Jul;108(3):363-8. doi: 10.1042/bj1080363.

The polysaccharide and mucopeptide components of the cell wall of Lactobacillus fermenti, serological group F, were separated by mild conditions of acid hydrolysis; the polysaccharide was composed of glucose and galactose. 2. Soluble cell-wall products were isolated from cell wall lysed by lysozyme and a Streptomyces enzyme preparation. The lysozyme-dissolved fraction contained a greater proportion of mucopeptide. 3. The soluble preparations were heated in dilute acid to hydrolyse the linkage between the polysaccharide and mucopeptide components and then incubated with acid phosphatase. 4. Inorganic phosphate was released from products of Streptomyces enzyme action but not from products of lysozyme action. 5. The phosphate was shown to be present in the mucopeptide as muramic acid phosphate. It is concluded that in the intact wall polysaccharide is joined to muramic acid by a phosphodiester linkage.

通过温和的酸水解条件分离出发酵乳杆菌F血清群细胞壁的多糖和粘肽成分；该多糖由葡萄糖和半乳糖组成。2. 从经溶菌酶和链霉菌酶制剂裂解的细胞壁中分离出可溶性细胞壁产物。溶菌酶溶解的部分含有更大比例的粘肽。3. 将可溶性制剂在稀酸中加热以水解多糖和粘肽成分之间的连接，然后与酸性磷酸酶一起孵育。4. 链霉菌酶作用的产物释放出无机磷酸盐，而溶菌酶作用的产物则未释放。5. 磷酸盐显示以磷酸胞壁酸的形式存在于粘肽中。得出的结论是，在完整的细胞壁中，多糖通过磷酸二酯键与胞壁酸相连。

Knox K W, Holmwood K J

Biochem J. 1968 Jul;108(3):363-8. doi: 10.1042/bj1080363.

The polysaccharide and mucopeptide components of the cell wall of Lactobacillus fermenti, serological group F, were separated by mild conditions of acid hydrolysis; the polysaccharide was composed of glucose and galactose. 2. Soluble cell-wall products were isolated from cell wall lysed by lysozyme and a Streptomyces enzyme preparation. The lysozyme-dissolved fraction contained a greater proportion of mucopeptide. 3. The soluble preparations were heated in dilute acid to hydrolyse the linkage between the polysaccharide and mucopeptide components and then incubated with acid phosphatase. 4. Inorganic phosphate was released from products of Streptomyces enzyme action but not from products of lysozyme action. 5. The phosphate was shown to be present in the mucopeptide as muramic acid phosphate. It is concluded that in the intact wall polysaccharide is joined to muramic acid by a phosphodiester linkage.

通过温和的酸水解条件分离出发酵乳杆菌F血清群细胞壁的多糖和粘肽成分；该多糖由葡萄糖和半乳糖组成。2. 从经溶菌酶和链霉菌酶制剂裂解的细胞壁中分离出可溶性细胞壁产物。溶菌酶溶解的部分含有更大比例的粘肽。3. 将可溶性制剂在稀酸中加热以水解多糖和粘肽成分之间的连接，然后与酸性磷酸酶一起孵育。4. 链霉菌酶作用的产物释放出无机磷酸盐，而溶菌酶作用的产物则未释放。5. 磷酸盐显示以磷酸胞壁酸的形式存在于粘肽中。得出的结论是，在完整的细胞壁中，多糖通过磷酸二酯键与胞壁酸相连。