Akutsu M, Suzuki K, Tsunoda S, Kano Y
Division of Medical Oncology and Clinical Pathology, Tochigi Cancer Center, Japan.
Gan To Kagaku Ryoho. 1996 Feb;23(3):297-302.
We studied the effects of carboplatin in combination with etoposide in human B-cell lymphoma cell lines, BALL-2, Dauji and human T-cell leukemia cell lines, CEM, HSB and MOLT-3 cells. Cells were incubated for 3 days in the presence of carboplatin and etoposide, and the combined drug and cell growth inhibition was determined by MTT assay. The effects of drug combinations at ID80 were analyzed by an improved isobologram method (Steel and Peckham). In the combination of carboplatin with etoposide, the data points fell in the envelope of additivity (additive effect) in all five cell lines. Synergistic and antagonistic effects were not observed. These findings suggest that the combination of carboplatin and etoposide are as effective as expected.
我们研究了卡铂联合依托泊苷对人B细胞淋巴瘤细胞系BALL-2、Dauji以及人T细胞白血病细胞系CEM、HSB和MOLT-3细胞的作用。将细胞在卡铂和依托泊苷存在的情况下孵育3天，通过MTT法测定联合用药及细胞生长抑制情况。采用改良的等效线图法（Steel和Peckham）分析药物组合在ID80时的作用。在卡铂与依托泊苷的联合用药中，所有五个细胞系的数据点均落在相加性（相加效应）范围内。未观察到协同和拮抗效应。这些发现表明卡铂和依托泊苷联合用药的效果与预期一致。
