Duterme O, Colinet D, Kummert J, Lepoivre P
Faculté Universitaire des Sciences Agronomiques, Laboratoire de Pathologie Végétale, Gembloux, Belgium.
Arch Virol. 1996;141(6):1067-75. doi: 10.1007/BF01718610.
The sequences of the N-terminal part of the coat protein cistron from six isolates of yam mosaic virus (YMV-TOG, YMV-COT, YMV-12, YMV-CAR, YMV-BU1 and YMV-BU2) were determined. The analysis of the deduced amino acid sequences revealed the presence of consensus motifs characteristic of the potyvirus genus supporting the classification of YMV as a potyvirus member. The alignment of the N-terminal part of the coat protein of YMV-TOG, YMV-COT, YMV-12 and YMV-CAR showed that they were identical in size (152 aa) while YMV-BU1 and YMV-BU2 were shorter (140 aa) due to a deletion of 12 aa. These amino acid sequences exhibit an overall sequence identity ranging from 70.4% to 97.4% while the identity level with the other potyviruses sequenced in the considered region is below 50%, confirming that YMV is a distinct member of the potyvirus genus. The detailed analysis of the amino acid sequence alignment and of the identity levels observed between the N-terminal part of the coat protein of the six YMV isolates lead us to suggest that they have to be considered as distantly related strains of YMV rather than closely related but distinct viruses.
测定了六种山药花叶病毒分离株（YMV - TOG、YMV - COT、YMV - 12、YMV - CAR、YMV - BU1和YMV - BU2）外壳蛋白顺反子N端部分的序列。对推导的氨基酸序列分析显示存在马铃薯Y病毒属特有的共有基序，支持将YMV分类为马铃薯Y病毒属成员。YMV - TOG、YMV - COT、YMV - 12和YMV - CAR外壳蛋白N端部分的比对表明，它们的大小相同（152个氨基酸），而YMV - BU1和YMV - BU2由于缺失12个氨基酸而较短（140个氨基酸）。这些氨基酸序列的总体序列同一性在70.4%至97.4%之间，而在所考虑区域与其他已测序马铃薯Y病毒的同一性水平低于50%，证实YMV是马铃薯Y病毒属的一个独特成员。对六种YMV分离株外壳蛋白N端部分氨基酸序列比对及观察到的同一性水平的详细分析使我们认为，应将它们视为YMV的远缘相关株系，而非亲缘关系密切但不同的病毒。
