Phalen D N, Wilson V G, Graham D L
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843, USA.
Avian Dis. 1996 Jan-Mar;40(1):140-9.
The glomerulopathy occurring in nestling nonbudgerigar parrots with avian polyomavirus (APV) disease was examined in 10 parrots. Glomerular lesions were characterized by the presence of dense, periodic acid-Schiff (PAS)-positive material that expanded the mesangium and that narrowed and at times occluded capillary lumina. PAS-staining was found to be more sensitive than hematoxylin and eosin in the detection of the lesions. Ultrastructurally, finely granular electron-dense material was found in massive intracapillary and mesangial condensates. Capillary endothelial cells exhibited changes consistent with cellular swelling. No evidence of chronic glomerular changes was observed. Immunofluorescent staining demonstrated that the PAS-positive, electron-dense condensates were complexes of IgG, avian polyomavirus antigen, and, in one bird, IgM. Viral DNA was detected in the serum of all six birds examined. Anti-APV antibodies were also present in all five serum samples examined. These findings suggested that the pathogenesis of this acute immune complex glomerulopathy and other APV-associated lesions depends on the presence of an appropriate ratio of circulating virus and anti-APV antibody.
对10只患有禽多瘤病毒（APV）病的雏非虎皮鹦鹉发生的肾小球病进行了检查。肾小球病变的特征是存在致密的、过碘酸希夫（PAS）染色阳性物质，该物质使系膜增宽，并使毛细血管腔变窄，有时甚至闭塞。发现PAS染色在检测病变方面比苏木精和伊红染色更敏感。超微结构上，在大量的毛细血管内和系膜冷凝物中发现了细颗粒状的电子致密物质。毛细血管内皮细胞表现出与细胞肿胀一致的变化。未观察到慢性肾小球变化的证据。免疫荧光染色显示，PAS阳性的电子致密冷凝物是IgG、禽多瘤病毒抗原的复合物，在一只鸟中还含有IgM。在所检查的所有6只鸟的血清中均检测到病毒DNA。在所检查的所有5份血清样本中也存在抗APV抗体。这些发现表明，这种急性免疫复合物性肾小球病和其他APV相关病变的发病机制取决于循环病毒和抗APV抗体的适当比例。
