Manolios N, Li Z G
Rheumatology Department, Royal North Shore Hospital, St. Leonards, Sydney, New South Wales, Australia.
Immunol Cell Biol. 1995 Dec;73(6):532-6. doi: 10.1038/icb.1995.83.
Selective pairwise interactions between a number of CD3 chains and the clonotypic T cell antigen receptor (TCR-alpha, -beta) chains have recently been reported. What still remains unanswered is the site of interaction between TCR-beta and CD3-gamma chains. To examine the region of interaction between TCR-beta and CD3-gamma chains, a variety of genetically altered TCR-beta and CD3-gamma chains were constructed using recombinant cDNA techniques. Non-T cells (COS-7) were transfected with cDNA constructs, metabolically labelled, and immunoprecipitates were analysed for assembly using non-equilibrium pH gel electrophoresis (NEPHGE)/SDS-PAGE. The results demonstrated that assembly between TCR-beta and CD3-gamma chains was localized to their extracellular domain. These findings, when coupled with the information on pairwise interactions and formation of higher order subcomplexes, extend our model of the structure of the TCR complex.
最近有报道称，多种CD3链与克隆型T细胞抗原受体（TCR-α、-β）链之间存在选择性的两两相互作用。然而，TCR-β与CD3-γ链之间的相互作用位点仍未得到解答。为了研究TCR-β与CD3-γ链之间的相互作用区域，利用重组cDNA技术构建了多种基因改造的TCR-β和CD3-γ链。用cDNA构建体转染非T细胞（COS-7），进行代谢标记，然后使用非平衡pH凝胶电泳（NEPHGE）/SDS-PAGE分析免疫沉淀产物的组装情况。结果表明，TCR-β与CD3-γ链之间的组装定位于它们的细胞外结构域。这些发现，结合关于两两相互作用和高阶亚复合物形成的信息，扩展了我们对TCR复合物结构的模型。
