Manolios N
Department of Rheumatology, Royal North Shore Hospital, Sydney, New South Wales, Australia.
Immunol Cell Biol. 1995 Dec;73(6):544-8. doi: 10.1038/icb.1995.85.
The formation of partial complexes in an African green monkey kidney cell line (COS cells) has been used to examine assembly of newly synthesized T cell antigen receptor (TCR) chains. The identification of assembly interactions between multiple subunits and the formation of higher order subcomplexes has led to the development of a model for the hierarchy of subunit assembly leading to a complete TCR. These assembly interactions suggest likely nearest neighbour relationships in the assembling structure and probably reflect the quarternary structure of the TCR complex. This may be important when examining the association of other molecules with TCR proteins or when trying to discern structural components involved with signal transduction.
在非洲绿猴肾细胞系（COS细胞）中形成部分复合物已被用于检测新合成的T细胞抗原受体（TCR）链的组装。对多个亚基之间组装相互作用的鉴定以及高阶亚复合物的形成，促成了一个关于亚基组装层次结构以形成完整TCR的模型的发展。这些组装相互作用表明了组装结构中可能的最近邻关系，并且可能反映了TCR复合物的四级结构。这在检查其他分子与TCR蛋白的关联时，或者在试图识别参与信号转导的结构成分时，可能是很重要的。
