Devlin A J, Danks J A, Faulkner M K, Power D M, Canario A V, Martin T J, Ingleton P M
Institute of Cancer Studies, Medical School, Sheffield, United Kingdom.
Gen Comp Endocrinol. 1996 Jan;101(1):83-90. doi: 10.1006/gcen.1996.0010.
Using antisera to regions of human parathyroid hormone-related protein (PTHrP) the saccus vasculosus (SV) of the sea bream (Sparus aurata) has been shown to contain immunoreactive PTHrP. By immunohistochemistry (IHC) the epithelial coronet cells in fixed and wax-embedded SV tissue reacted with antisera to the prepro region of human PTHrP (-13 to +2), the N-terminus PTHrP (1-16), and the midmolecule PTHrP (50-69). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of saccus extracts and incubation media contained two major proteins of 14.3 and 15 kDa. By Western blotting these two proteins both reacted with the three antisera used for IHC, suggesting that they are immunochemically similar to human PTHrP (1-84). Ultrastructurally the coronet cells of Sparus saccus vasculosus resembled coronet cells described for other teleosts, with an abundant smooth endoplasmic reticulum (SER) which was more highly organized in the coronets. IHC at EM level showed reaction mainly with the membranes of the SER. These results suggest that S. aurata saccus vasculosus may produce a PTHrP-like molecule similar to human PTHrP.
利用针对人甲状旁腺激素相关蛋白（PTHrP）各区域的抗血清，已证实海鲷（Sparus aurata）的血管囊（SV）含有免疫反应性PTHrP。通过免疫组织化学（IHC）方法，固定并包埋于石蜡中的SV组织中的上皮冠状细胞与针对人PTHrP前原区域（-13至+2）、PTHrP N端（1-16）和PTHrP中分子区域（50-69）的抗血清发生反应。血管囊提取物和孵育培养基的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示有两种主要蛋白质，分子量分别为14.3 kDa和15 kDa。通过蛋白质印迹法，这两种蛋白质均与用于IHC的三种抗血清发生反应，表明它们在免疫化学上与人PTHrP（1-84）相似。在超微结构上，海鲷血管囊的冠状细胞类似于其他硬骨鱼所描述的冠状细胞，具有丰富的滑面内质网（SER），在冠状细胞中其组织更为高度有序。电子显微镜水平的免疫组织化学显示反应主要发生在滑面内质网的膜上。这些结果表明，海鲷血管囊可能产生一种与人PTHrP相似的PTHrP样分子。
